BRENDA - Enzyme Database
show all sequences of 1.12.98.4

Purification and characterization of sulfur reductase from a moderately thermophilic bacterial strain, TI-1, that oxidizes iron

Sugio, T.; Oda, K.; Matsumoto, K.; Takai, M.; Wakasa, S.; Kamimura, K.; Biosci. Biotechnol. Biochem. 62, 705-709 (1998)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
8-hydroxyquinoline
-
thermophilic iron-oxidizing bacterium strain TI-1
ethylenediaminetetraacetic acid
-
thermophilic iron-oxidizing bacterium strain TI-1
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
thermophilic iron-oxidizing bacterium strain TI-1
iodoacetic acid
-
thermophilic iron-oxidizing bacterium strain TI-1
N-ethylmaleimide
-
thermophilic iron-oxidizing bacterium strain TI-1
potassium tetrathionate
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium dithionate
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium sulfite
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium thiosulfate
-
thermophilic iron-oxidizing bacterium strain TI-1
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
thermophilic iron-oxidizing bacterium strain TI-1
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
86000
-
gel-filtration
thermophilic iron-oxidizing bacterium strain TI-1
Organism
Organism
UniProt
Commentary
Textmining
thermophilic iron-oxidizing bacterium strain TI-1
-
production of H2S, when grown in a medium containing ferrous ions, elemental sulfur and L-glutamic acid
-
Purification (Commentary)
Purification (Commentary)
Organism
-
thermophilic iron-oxidizing bacterium strain TI-1
Reaction
Reaction
Commentary
Organism
Reaction ID
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1
two enzymes are involved in the production of H2S: the sulfur reductase and the thiosulfate reductase
thermophilic iron-oxidizing bacterium strain TI-1
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.73
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
no substrates are sulfite, thiosulfate, tetrathionate
437779
thermophilic iron-oxidizing bacterium strain TI-1
?
-
-
-
?
sulfur + electron donor
electron donor: NADH, NADPH not
437779
thermophilic iron-oxidizing bacterium strain TI-1
H2S + oxidized electron donor
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 48000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
70
-
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
100% activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
70
-
73% loss of activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
80
-
88% loss of activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
thermophilic iron-oxidizing bacterium strain TI-1
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
10
complete loss of activity below pH 5
thermophilic iron-oxidizing bacterium strain TI-1
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
8-hydroxyquinoline
-
thermophilic iron-oxidizing bacterium strain TI-1
ethylenediaminetetraacetic acid
-
thermophilic iron-oxidizing bacterium strain TI-1
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
thermophilic iron-oxidizing bacterium strain TI-1
iodoacetic acid
-
thermophilic iron-oxidizing bacterium strain TI-1
N-ethylmaleimide
-
thermophilic iron-oxidizing bacterium strain TI-1
potassium tetrathionate
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium dithionate
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium sulfite
-
thermophilic iron-oxidizing bacterium strain TI-1
sodium thiosulfate
-
thermophilic iron-oxidizing bacterium strain TI-1
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
thermophilic iron-oxidizing bacterium strain TI-1
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
86000
-
gel-filtration
thermophilic iron-oxidizing bacterium strain TI-1
Purification (Commentary) (protein specific)
Commentary
Organism
-
thermophilic iron-oxidizing bacterium strain TI-1
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.73
-
-
thermophilic iron-oxidizing bacterium strain TI-1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
no substrates are sulfite, thiosulfate, tetrathionate
437779
thermophilic iron-oxidizing bacterium strain TI-1
?
-
-
-
?
sulfur + electron donor
electron donor: NADH, NADPH not
437779
thermophilic iron-oxidizing bacterium strain TI-1
H2S + oxidized electron donor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 48000, SDS-PAGE
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
70
-
thermophilic iron-oxidizing bacterium strain TI-1
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
100% activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
70
-
73% loss of activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
80
-
88% loss of activity for 30 min
thermophilic iron-oxidizing bacterium strain TI-1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
thermophilic iron-oxidizing bacterium strain TI-1
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
10
complete loss of activity below pH 5
thermophilic iron-oxidizing bacterium strain TI-1
Other publictions for EC 1.12.98.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716255
Yang
SurR regulates hydrogen produc ...
Pyrococcus furiosus
Mol. Microbiol.
77
1111-1122
2010
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1
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1
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674514
Guiral
A membrane-bound multienzyme, ...
Aquifex aeolicus
J. Biol. Chem.
280
42004-42015
2005
1
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659878
Laska
Membrane-bound hydrogenase and ...
Acidianus ambivalens
Microbiology
149
2357-2371
2003
-
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1
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3
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9
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1
1
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437780
Keller
Hydrogen-sulfur oxidoreductase ...
Pyrodictium abyssi
Methods Enzymol.
331
442-451
2001
1
-
-
-
-
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4
1
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1
1
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1
1
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4
1
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1
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1
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1
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1
1
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437781
Laska
Improved purification of the m ...
Acidianus ambivalens
J. Chromatogr. B
737
151-160
2000
-
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437782
Ng
Purification and some properti ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans NASF-1
J. Biosci. Bioeng.
90
199-203
2000
-
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1
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10
1
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1
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439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
-
-
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1
2
5
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7
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1
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5
1
2
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5
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5
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5
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5
1
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437773
Klimmek
The single cysteine residue of ...
Wolinella succinogenes
Eur. J. Biochem.
263
79-84
1999
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1
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1
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5
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745391
Silva
On the prosthetic groups of t ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
4
284-291
1999
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745603
Selvaggi
-
In vitro hydrogen photoproduc ...
Pyrococcus furiosus
J. Photochem. Photobiol. A
125
107-112
1999
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1
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437778
Dirmeier
Purification and properties of ...
Pyrodictium abyssi
Eur. J. Biochem.
252
486-491
1998
1
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437779
Sugio
Purification and characterizat ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
62
705-709
1998
2
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7
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1
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675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
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437776
Arendsen
Redox properties of the sulfhy ...
Pyrococcus furiosus
FEBS Lett.
368
117-121
1995
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723118
Pedroni
Characterization of the locus ...
Pyrococcus furiosus
Microbiology
141
449-458
1995
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437774
Fauque
-
Sulfur reductases from spirill ...
Sulfurospirillum deleyianum, Wolinella succinogenes
Methods Enzymol.
243
367-383
1994
3
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1
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437775
Jankielewicz
-
Polysulfide reductase and form ...
Wolinella succinogenes
Arch. Microbiol.
162
238-242
1994
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437777
Childers
Characterization and regulatio ...
Thermotoga neapolitana
Appl. Environ. Microbiol.
60
2622-2626
1994
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1
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722289
Ma
-
Hydrogen production from pyruv ...
Pyrococcus furiosus
FEMS Microbiol. Lett.
122
245-250
1994
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4
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1
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1
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4
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