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Literature summary for 1.12.98.2 extracted from
- Purification, properties and primary structure of H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanococcus thermolithotrophicus (1996), Arch. Microbiol., 165, 187-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
General Stability
| General Stability | Organism |
|---|---|
| 50% loss of activity after freezing and thawing | Methanothermococcus thermolithotrophicus |
| the rate of inactivation increases upon dilution and in the presence of O2 and decreases upon the addition of 5 mg/ml albumin | Methanothermococcus thermolithotrophicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
N5,N10-methylenetetrahydromethanopterin | - |
Methanothermococcus thermolithotrophicus |  |
| 0.15 | - |
H2 | - |
Methanothermococcus thermolithotrophicus | |
| 0.85 | - |
N5,N10-methenyltetrahydromethanopterin | - |
Methanothermococcus thermolithotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not contain nickel or iron/sulfur clusters | Methanothermobacter thermautotrophicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37992 | - |
4 * 37992, calculation from nucleotide sequence | Methanothermococcus thermolithotrophicus |
| 38038 | - |
4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage | Methanothermococcus thermolithotrophicus |
| 43000 | - |
4 * 43000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
| 150000 | - |
gel filtration | Methanothermococcus thermolithotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermococcus thermolithotrophicus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| purified enzyme is very labile even under strictly anoxic conditions | Methanothermococcus thermolithotrophicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 440 | - |
- |
Methanothermococcus thermolithotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N5,N10-methylenetetrahydromethanopterin + H+ | r | Methanothermobacter thermautotrophicus | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? | |
| N5,N10-methylenetetrahydromethanopterin + H+ | r | Methanothermococcus thermolithotrophicus | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
| tetramer | 4 * 37992, calculation from nucleotide sequence | Methanothermococcus thermolithotrophicus |
| tetramer | 4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage | Methanothermococcus thermolithotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Methanothermococcus thermolithotrophicus |
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