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Literature summary for 1.12.98.2 extracted from

  • Zirngibl, C.; Hedderich, R.; Thauer, R.K.
    N5,N10-Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity (1990), FEBS Lett., 261, 112-116.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
H2 inhibits formation of N5,N10-methenyltetrahydromethanopterin Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
N5,N10-methylenetetrahydromethanopterin
-
Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains approximately 1 mol of iron per mol of enzyme Methanothermobacter thermautotrophicus
Nickel contains less than 0.1 mol Ni per mol of enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Methanothermobacter thermautotrophicus
45000
-
gradient PAGE Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
750
-
-
Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability Organism
4°C, pH 7.6, 50 mM Tris-HCl, 20% glycerol, under N2 gas phase, 50% loss of activity after 1 week Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ no reaction with viologen dyes Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ no reaction with viologen dyes Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
?

Subunits

Subunits Comment Organism
monomer x * 43000, SDS-PAGE Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Methanothermobacter thermautotrophicus