Literature summary for 1.12.98.1 extracted from

Livingston, D.J.; Fox, J.A.; Orme-Johnson, W.H.; Walsh, C.T.
8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 2. Kinetic and hydrogen-transfer studies (1987), Biochemistry, 26, 4228-4237.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Procion Blue HB	stimulates methyl viologen reduction	Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors	Comment	Organism	Structure
CO	dead end inhibitor, competitive for H2, uncompetetitive versus F0	Methanothermobacter thermautotrophicus
coenzyme F0	substrate inhibition above 0.05 mM	Methanothermobacter thermautotrophicus
Procion Blue HB	inhibits F0 reduction	Methanothermobacter thermautotrophicus
reduced coenzyme F0	noncompetitive product inhibition	Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	H2	-	Methanothermobacter thermautotrophicus
0.026	-	coenzyme F0	-	Methanothermobacter thermautotrophicus
0.036	-	coenzyme F420	-	Methanothermobacter thermautotrophicus
0.1	-	reduced coenzyme F0	anaerobic conditions	Methanothermobacter thermautotrophicus
1.5	-	methyl viologen	-	Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	iron-sulfur cluster	Methanothermobacter thermautotrophicus
Iron	iron-sulfur cluster	Methanococcus voltae
Nickel	-	Methanothermobacter thermautotrophicus
Nickel	-	Methanococcus voltae
sulfur	iron-sulfur cluster	Methanothermobacter thermautotrophicus
sulfur	iron-sulfur cluster	Methanococcus voltae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + coenzyme F420	Methanothermobacter thermautotrophicus	enzyme of methanogenesis pathway	reduced coenzyme F420	-	?
H2 + coenzyme F420	Methanococcus voltae	enzyme of methanogenesis pathway	reduced coenzyme F420	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanococcus voltae	-	-	-
Methanothermobacter thermautotrophicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + coenzyme F0	-	Methanothermobacter thermautotrophicus	reduced coenzyme F0	-	r
H2 + coenzyme F420	-	Methanothermobacter thermautotrophicus	reduced coenzyme F420	-	?
H2 + coenzyme F420	-	Methanococcus voltae	reduced coenzyme F420	-	?
H2 + coenzyme F420	enzyme of methanogenesis pathway	Methanothermobacter thermautotrophicus	reduced coenzyme F420	-	?
H2 + coenzyme F420	enzyme of methanogenesis pathway	Methanococcus voltae	reduced coenzyme F420	-	?
H2 + methyl viologen	-	Methanothermobacter thermautotrophicus	?	-	?
H2 + methyl viologen	-	Methanococcus voltae	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2050	-	coenzyme F420	-	Methanothermobacter thermautotrophicus
2250	-	methyl viologen	-	Methanothermobacter thermautotrophicus
2250	-	coenzyme F0	equal turnover in reverse reaction under anaerobic conditions	Methanothermobacter thermautotrophicus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Methanothermobacter thermautotrophicus
FAD	-	Methanococcus voltae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.087	-	coenzyme F0	-	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)