Literature summary for 1.12.98.1 extracted from

Jacobson, F.S.; Daniels, L.; Fox, J.A.; Walsh, C.T.; Orme-Johnson, W.H.
Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum (1982), J. Biol. Chem., 257, 3385-3388.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	H2	+ methyl viologen, pH 9	Methanothermobacter thermautotrophicus
0.01	-	H2	+ coenzyme F0, pH 7.2	Methanothermobacter thermautotrophicus
0.019	-	coenzyme F420	pH 7.2	Methanothermobacter thermautotrophicus
0.034	-	coenzyme F0	pH 7.2	Methanothermobacter thermautotrophicus
0.42	-	methyl viologen	pH 9	Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	-	Methanothermobacter thermautotrophicus
sulfur	-	Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
500000	-	native enzyme bigger than 500000	Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + coenzyme F420	Methanothermobacter thermautotrophicus	enzyme of methanogenesis pathway	reduced coenzyme F420	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + coenzyme F0	F0 is 7,8-didemethyl-8-hydroxy-5-deazariboflavin	Methanothermobacter thermautotrophicus	reduced coenzyme F0	-	?
H2 + coenzyme F420	F420 is 7,8-didemethyl-8-hydroxy-5-deazaflavin	Methanothermobacter thermautotrophicus	reduced coenzyme F420	-	?
H2 + coenzyme F420	enzyme of methanogenesis pathway	Methanothermobacter thermautotrophicus	reduced coenzyme F420	-	?
H2 + methyl viologen	-	Methanothermobacter thermautotrophicus	?	-	?

Subunits

Subunits	Comment	Organism
multimer	alpha,beta,gamma 40000 + 31000 + 26000, SDS-PAGE, exact subunit composition not known, ratio 2:2:1 leading to MW 170000	Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
725	-	methyl viologen	pH 7.2	Methanothermobacter thermautotrophicus
725	-	coenzyme F420	pH 7.2	Methanothermobacter thermautotrophicus
725	-	coenzyme F0	pH 7.2	Methanothermobacter thermautotrophicus
4000	-	methyl viologen	pH 9	Methanothermobacter thermautotrophicus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	2.3 mol per mol 170000 Da unit	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)