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Literature summary for 1.12.7.2 extracted from

  • Kim, E.; Tong, X.; Lee, J.
    The ferredoxin Rr-HydB is required for the H2-evolving activity of Rr-HydA, a [FeFe]-hydrogenase of Rhodospirillum rubrum (2015), Int. J. Hydrogen Energy, 40, 4320-4328 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus(DE3) cells and in a Rhodobacter sphaeroides nifDK hupSL mutant Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized ferredoxin Rhodospirillum rubrum
-
reduced ferredoxin + H+
-
?
H2 + oxidized ferredoxin Rhodospirillum rubrum UR1
-
reduced ferredoxin + H+
-
?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-
Rhodospirillum rubrum UR1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin-Sepharose column chromatography and Superdex 200 gel filtration Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + methyl viologen
-
Rhodospirillum rubrum reduced methyl viologen + H+
-
?
H2 + methyl viologen
-
Rhodospirillum rubrum UR1 reduced methyl viologen + H+
-
?
H2 + oxidized ferredoxin
-
Rhodospirillum rubrum reduced ferredoxin + H+
-
?
H2 + oxidized ferredoxin
-
Rhodospirillum rubrum UR1 reduced ferredoxin + H+
-
?
H2 + oxidized spinach ferredoxin
-
Rhodospirillum rubrum reduced spinach ferredoxin + H+
-
?
H2 + oxidized spinach ferredoxin
-
Rhodospirillum rubrum UR1 reduced spinach ferredoxin + H+
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Rhodospirillum rubrum

Synonyms

Synonyms Comment Organism
hydA
-
Rhodospirillum rubrum
[FeFe]-hydrogenase
-
Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
[Fe-S]-center
-
Rhodospirillum rubrum