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Literature summary for 1.12.7.2 extracted from

  • Chen, J.S.; Blanchard, D.K.
    Isolation and properties of a unidirectional H2-oxidizing hydrogenase from the strictly anaerobic N2-fixing bacterium Clostridium pasteurianum W5 (1978), Biochem. Biophys. Res. Commun., 84, 1144-1150.
    View publication on PubMed
Search for more literature for 1.12.7.2

Inhibitors

Inhibitors Comment Organism Structure
CO at 0.5 atm 98% inhibition Clostridium pasteurianum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm outside the cell membrane Clostridium pasteurianum 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized ferredoxin Clostridium pasteurianum
-
 H+ + reduced ferredoxin
-
 r
H2 + oxidized ferredoxin Clostridium pasteurianum W5
-
 H+ + reduced ferredoxin
-
 r

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-
Clostridium pasteurianum
-
 hydrogenase II, uptake hydrogenase
-
Clostridium pasteurianum W5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
400
-
 hydrogen oxidation, methyl viologen as electron acceptor Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor methyl viologen as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor benzyl viologen as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor dichlorophenol indophenol as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor methyl viologen as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor benzyl viologen as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
H2 + electron acceptor dichlorophenol indophenol as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
H2 + oxidized ferredoxin
-
 Clostridium pasteurianum H+ + reduced ferredoxin
-
 r
H2 + oxidized ferredoxin
-
 Clostridium pasteurianum W5 H+ + reduced ferredoxin
-
 r