Literature summary for 1.12.7.2 extracted from

Dobrindt, U.; Blaut, M.
Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport (1996), Arch. Microbiol., 165, 141-147.

Search for more literature for 1.12.7.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.341	-	H2	-	Sporomusa sphaeroides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	cytoplasmic	Sporomusa sphaeroides	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	15.6 mol iron per mol enzyme	Sporomusa sphaeroides
Ni	0.6 mol per mol enzyme	Sporomusa sphaeroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	1 * 65000 + 1 * 37000, SDS-PAGE	Sporomusa sphaeroides
65000	-	1 * 65000 + 1 * 37000, SDS-PAGE	Sporomusa sphaeroides
122000	-	native PAGE	Sporomusa sphaeroides
130000	-	gel filtration	Sporomusa sphaeroides

Organism

Organism	UniProt	Comment	Textmining
Sporomusa sphaeroides	-	strain DSM 2875	-

Purification (Commentary)

Purification (Comment)	Organism
purified under aerobic conditions, requires reductive activation	Sporomusa sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
19.8	-	hydrogen oxidation, benzyl viologen as electron acceptor	Sporomusa sphaeroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + electron acceptor	cytochrome b as electron acceptor	Sporomusa sphaeroides	H+ + reduced electron acceptor	-	r
H2 + electron acceptor	methyl viologen as electron acceptor	Sporomusa sphaeroides	H+ + reduced electron acceptor	-	r
H2 + electron acceptor	benzyl viologen as electron acceptor	Sporomusa sphaeroides	H+ + reduced electron acceptor	-	r

Subunits

Subunits	Comment	Organism
dimer	1 * 65000 + 1 * 37000, SDS-PAGE	Sporomusa sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Sporomusa sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.683	-	H2	-	Sporomusa sphaeroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Sporomusa sphaeroides

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Release 2023.1 (January 2023)