Literature summary for 1.12.5.1 extracted from

Dross, F.; Geisler, V.; Lenger, R.; Theis, F.; Krafft, T.; Fahrenholz, F.; Kojro, E.; Duchene, A.; Tripier, D.; Juvenal, K.; Krueger, A.
The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes (1992), Eur. J. Biochem., 206, 93-102.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Wolinella succinogenes

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasmic membrane	-	Wolinella succinogenes	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	x * 23000, cytochrome b, + x * 30000 + x * 60000, SDS-PAGE	Wolinella succinogenes
26828	-	x * 26828, HydC, + x * 31968, HydA, + x * 63866, HydB, calculation from nucleotide sequence	Wolinella succinogenes
30000	-	x * 23000, cytochrome b, + x * 30000 + x * 60000, SDS-PAGE	Wolinella succinogenes
31968	-	x * 26828, HydC, + x * 31968, HydA, + x * 63866, HydB, calculation from nucleotide sequence	Wolinella succinogenes
60000	-	x * 23000, cytochrome b, + x * 30000 + x * 60000, SDS-PAGE	Wolinella succinogenes
63866	-	x * 26828, HydC, + x * 31968, HydA, + x * 63866, HydB, calculation from nucleotide sequence	Wolinella succinogenes

Organism

Organism	UniProt	Comment	Textmining
Wolinella succinogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Wolinella succinogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
505	-	-	Wolinella succinogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + 2,3-dimethyl-1,4-naphthoquinone	a hydrophilic analogue of menaquinone	Wolinella succinogenes	reduced 2,3-dimethyl-1,4-naphthoquinone	-	?
H2 + benzyl viologen	-	Wolinella succinogenes	reduced benzyl viologen	-	?

Subunits

Subunits	Comment	Organism
?	x * 23000, cytochrome b, + x * 30000 + x * 60000, SDS-PAGE	Wolinella succinogenes
?	x * 26828, HydC, + x * 31968, HydA, + x * 63866, HydB, calculation from nucleotide sequence	Wolinella succinogenes

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c	contains 0.0064 mM per g of protein, which is reduced upon the addition of H2	Wolinella succinogenes

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Release 2023.1 (January 2023)