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Literature summary for 1.12.2.1 extracted from
- Purification and properties of the membrane-bound by hydrogenase from Desulfovibrio desulfuricans (1983), Biochem. J., 209, 445-454.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO | - |
Desulfovibrio desulfuricans |  |
| KI | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
| N-bromosuccinimide | 0.07 mM, complete loss of activity | Desulfovibrio desulfuricans | |
| NaCl | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
| NaF | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
| O2 | strong inhibition of H2 evolution from reduced methyl viologen | Desulfovibrio desulfuricans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
ferrocytochrome c3 | H2-evolution assay | Desulfovibrio desulfuricans | |
| 0.003 | - |
reduced methyl viologen | H2-evolution assay | Desulfovibrio desulfuricans | |
| 0.08 | - |
reduced benzyl viologen | H2-evolution assay | Desulfovibrio desulfuricans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to | Desulfovibrio desulfuricans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 6 iron atoms and six acid-labile sulfur groups per molecule, presence of [4Fe-4S]3+ or [3Fe-3S]3+ | Desulfovibrio desulfuricans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Desulfovibrio desulfuricans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Desulfovibrio desulfuricans | cytochrome c3 is the natural electron acceptor | ? | - |
? | |
| additional information | Desulfovibrio desulfuricans Norway | cytochrome c3 is the natural electron acceptor | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio desulfuricans | - |
Norway | - |
| Desulfovibrio desulfuricans Norway | - |
Norway | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane-bound enzyme | Desulfovibrio desulfuricans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Desulfovibrio desulfuricans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, atmosphere of N2, air or H2, very little loss of activity after 4 weeks | Desulfovibrio desulfuricans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H+ + neutral red | - |
Desulfovibrio desulfuricans | H2 + oxidized neutral red | - |
? | |
| H+ + neutral red | - |
Desulfovibrio desulfuricans Norway | H2 + oxidized neutral red | - |
? | |
| H+ + phenosafranine | - |
Desulfovibrio desulfuricans | H2 + oxidized phenosafranine | - |
? | |
| H+ + reduced methyl viologen | weak activity in H2-uptake assay | Desulfovibrio desulfuricans | H2 + methyl viologen | - |
r | |
| H2 + benzyl viologen | - |
Desulfovibrio desulfuricans | H+ + reduced benzyl viologen | - |
r | |
| H2 + benzyl viologen | - |
Desulfovibrio desulfuricans Norway | H+ + reduced benzyl viologen | - |
r | |
| H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans | H+ + ferrocytochrome c3 | - |
r | |
| H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans Norway | H+ + ferrocytochrome c3 | - |
r | |
| H2 + methylene blue | no activity in H2-evolution assay | Desulfovibrio desulfuricans | H+ + reduced methylene blue | - |
ir | |
| H2 + methylene blue | no activity in H2-evolution assay | Desulfovibrio desulfuricans Norway | H+ + reduced methylene blue | - |
ir | |
| additional information | cytochrome c3 is the natural electron acceptor | Desulfovibrio desulfuricans | ? | - |
? | |
| additional information | cytochrome c3 is the natural electron acceptor | Desulfovibrio desulfuricans Norway | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Desulfovibrio desulfuricans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
4 weeks, atmosphere of N2, air or H2, very little loss of activity | Desulfovibrio desulfuricans |
| 23 | - |
atmosphere of N2, air or H2, 40% loss of activity after 2 weeks, complete loss of activity after 4 weeks | Desulfovibrio desulfuricans |
| 77 | - |
stable below, 2 h | Desulfovibrio desulfuricans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
H2-evolution assay | Desulfovibrio desulfuricans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity | Desulfovibrio desulfuricans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c3 | - |
Desulfovibrio desulfuricans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki for CO is 1.64 kPa | Desulfovibrio desulfuricans |
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