Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme is efficient in vitro biohydrogen production | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 1 nickel-iron catalytic site and 6 iron-sulfur clusters, contains 23 iron atoms/heterotetramer | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Pyrococcus furiosus COM1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-tag II column chromatography | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.23 | - |
crude native enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C | Pyrococcus furiosus |
7.96 | - |
crude recombinant enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C | Pyrococcus furiosus |
126 | - |
recombinant enzyme after 24fold purification, using methyl viologen as cosubstrate, at pH 8.4 and 80°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + NADPH | - |
Pyrococcus furiosus | H2 + NADP+ | - |
r | |
H+ + NADPH | - |
Pyrococcus furiosus COM1 | H2 + NADP+ | - |
r | |
H+ + reduced methyl viologen | - |
Pyrococcus furiosus | H2 + oxidized methyl viologen | - |
? | |
H+ + reduced methyl viologen | - |
Pyrococcus furiosus COM1 | H2 + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
SHI | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | the native enzyme shows half-lives of 14 h at 90°C under argon (H2 evolution), 21 h at 25°C under air (H2 evolution), and 10 h at 90°C under argon (H2 oxidation). The recombinant enzyme shows half-lives of 6 h at 90°C under argon (H2 evolution), 25 h at 25°C under air (H2 evolution), and 5 h at 90°C under argon (H2 oxidation) | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Pyrococcus furiosus | |
NADPH | dependent on | Pyrococcus furiosus | |
[2Fe-2S]-center | contains 1 [2Fe-2S] centre | Pyrococcus furiosus | |
[4Fe-4S]-center | contains 2 [4Fe-4S] centres | Pyrococcus furiosus |