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Literature summary for 1.12.1.3 extracted from
- A recombinant 12-His tagged Pyrococcus furiosus soluble [NiFe]-hydrogenase I overexpressed in Thermococcus kodakarensis KOD1 facilitates hydrogen-powered in vitro NADH regeneration (2019), Biotechnol. J., 14, e1800301 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the study provides a cost-efficient method to obtain hyperthermostable hydrogenases, which can be used in in vitro synthetic enzymatic biosystems for cofactor regeneration and hydrogen production. It has great potential in biocatalysis, bioelectrochemistry, and clean energy production | Pyrococcus furiosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Thermococcus kodakarensis KOD1 results in more than 1200-fold enhancement in the hydrogenase activity of the cell lysate compared to that of the host strain with an empty vector | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + NADP+ | Pyrococcus furiosus | no activity with NAD+ | H+ + NADPH | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| an active, purified 12-His tagged recombinant SHI (rSHI) is obtained by one-step affinity adsorption on nickel-charged resin | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + NADP+ | no activity with NAD+ | Pyrococcus furiosus | H+ + NADPH | - |
? | |
| oxidized benzyl viologen + H2 | - |
Pyrococcus furiosus | reduced benzyl viologen + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | - |
Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SHI | - |
Pyrococcus furiosus |
| [NiFe]-hydrogenase I | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | 60°C: about 40% of maximal activity, 90°C: about 80% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life of recombinant enzyme: 70 h | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Pyrococcus furiosus | |
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