Literature summary for 1.12.1.3 extracted from

Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.
High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form (2015), Protein Expr. Purif., 107, 90-94.

Search for more literature for 1.12.1.3

Application

Application	Comment	Organism
synthesis	biohydrogen production from sugars using a mixture of enzymes in an in vitro cell-free synthetic pathway. Development of this process at an industrial scale is limited by the availability of the H2-producing enzyme	Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment)	Organism
the four gene operon encoding the enzyme is overexpressed in Pyrococcus furiosus and includes a polyhistidine affinity tag	Pyrococcus furiosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Pyrococcus furiosus	5737	-

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q8U2E5 and Q8U2E4 and E7FHU4 and E7FI44	Q8U2E5: gene PF0891, Q8U2E4: gene PF0892, E7FHU4: gene PF0893, E7FI44: gene PF0894	-

Purification (Commentary)

Purification (Comment)	Organism
one-step purification of the recombinant enzyme including a polyhistidine affinity tag results in a 50fold increase in yield compared to the four-step purification procedure for the native enzyme	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADPH + H+	-	Pyrococcus furiosus	H2 + NADP+	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	a maturation pathway is proposed where, in the terminal step, the NiFe-containing catalytic subunit assembles with NADPH-oxidizing trimeric form to give the active holoenzym	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
[NiFe]-hydrogenase	-	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	assay at	Pyrococcus furiosus

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Release 2023.1 (January 2023)