Cloned (Comment) | Organism |
---|---|
expression of C-terminal domain of subunit HndA and N-terminal domain of subunit HndD in Escherichia coli | Solidesulfovibrio fructosivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the HndA subunit belongs to the [2Fe-2S] ferredoxin family. HndB is a FeS subunit | Solidesulfovibrio fructosivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solidesulfovibrio fructosivorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
C-terminal domain of subunit HndA and N-terminal domain of subunit HndD recombinantly expressed in Escherichia coli | Solidesulfovibrio fructosivorans |
Subunits | Comment | Organism |
---|---|---|
tetramer | the HndC subunit (52 kDa) corresponds to the NADP-reducing unit, and the HndD subunit (63.5 kDa) is homologous to Clostridium pasteurianum hydrogenase. The role of HndA and HndB subunits (18.8 kDa and 13.8 kDa, respectively) in the complex remains unknown. HndAc and HndB can form a heterodimeric intermediate in the electron transfer between the hydrogenase (HndD) active site and the NADP reduction site in HndC | Solidesulfovibrio fructosivorans |