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Literature summary for 1.12.1.2 extracted from

  • Aggag, M.; Schlegel, H.G.
    Studies on a gram-positive hydrogen bacterium, Nocardia opaca 1 b. III. Purification, stability and some properties of the soluble hydrogen dehydrogenase (1974), Arch. Microbiol., 100, 25-39.
    View publication on PubMed
Search for more literature for 1.12.1.2

Activating Compound

Activating Compound Comment Organism Structure
glutathione up to 0.1 mM: activation, above 0.1 mM: inactivation Rhodococcus opacus
NH4+ activates Rhodococcus opacus
phosphate increase in NAD-reduction rate Rhodococcus opacus
SO42- activates Rhodococcus opacus

General Stability

General Stability Organism
5 mM Mg2+ and 0.5 mM Ni2+ stabilizes Rhodococcus opacus
addition of 5 mM Mg2+ + 0.5 mM Ni2+ stabilizes Rhodococcus opacus

Inhibitors

Inhibitors Comment Organism Structure
cyanide 1 mM, 33% inhibition Rhodococcus opacus
glutathione above 0.1 mM: inactivation, up to 0.1 mM: activation Rhodococcus opacus
iodoacetate 100 mM, 36% inhibition Rhodococcus opacus
Na+
-
 Rhodococcus opacus
p-chloromercuribenzoate 0.02 mM, complete inhibition, reversed by addition of glutathione Rhodococcus opacus
Sulfide 0.1 mM, 10% inhibition Rhodococcus opacus
Triethanolamine buffer at high concentration Rhodococcus opacus
Tris-buffer at high concentration Rhodococcus opacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 H2
-
 Rhodococcus opacus
0.123
-
 NAD+
-
 Rhodococcus opacus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Rhodococcus opacus
Mg2+ NAD-reduction: no linear kinetics in absence of metals, 0.5 mM Ni2+ and 5 mM Mg2+ required Rhodococcus opacus
Ni2+ NAD-reduction: no linear kinetics in absence of metals, 0.5 mM Ni2+ and 5 mM Mg2+ required Rhodococcus opacus

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-
Rhodococcus opacus 1B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200 Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103
-
-
 Rhodococcus opacus

Storage Stability

Storage Stability Organism
-20°C, 4 mg/ml protein concentration, pH 7.0 Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+ artificial electron acceptor benzyl viologen Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methyl viologen Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+ electron acceptor NAD+ Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methylene blue Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor benzyl viologen Rhodococcus opacus 1B H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methyl viologen Rhodococcus opacus 1B H+ + NADH
-
 ?
H2 + NAD+ electron acceptor NAD+ Rhodococcus opacus 1B H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methylene blue, only in presence of catalytic amounts of NAD+ Rhodococcus opacus 1B H+ + NADH
-
 ?
H2 + NAD+ artificial electron acceptor methylene blue Rhodococcus opacus 1B H+ + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 enzyme prepared in Tris or triethanolamine buffer: 90% loss of activity after 10 min, enzyme prepared in K-phosphate buffer: 30% loss of activity after 10 min Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 methylene blue reduction, catalytic amounts of NAD+ required Rhodococcus opacus
8 8.2 electron acceptor NAD+ Rhodococcus opacus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 triethanolamine buffer, Tris buffer pH 7.0: 40-45% activity, triethanolamine buffer, Tris buffer pH 9.5: 60-75% activity Rhodococcus opacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 highest stability, 4°C, H2-atmosphere, 3 days Rhodococcus opacus

Cofactor

Cofactor Comment Organism Structure
flavin only when FMN or riboflavin are added in combination with Mg2+ or Ni2+, NAD-reducing system is activated Rhodococcus opacus