BRENDA - Enzyme Database
show all sequences of 1.12.1.2

Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16

Schneider, K.; Schlegel, H.G.; Biochim. Biophys. Acta 452, 66-80 (1976)

Data extracted from this reference:

General Stability
General Stability
Organism
unstable in reduced active form
Cupriavidus necator
Inhibitors
Inhibitors
Commentary
Organism
Structure
high ionic strength
200 mM phosphate buffer, 70% inhibition
Cupriavidus necator
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
H2
-
Cupriavidus necator
0.123
-
NAD+
-
Cupriavidus necator
0.56
-
NAD+
-
Cupriavidus necator
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Cupriavidus necator
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+; non-heme iron protein
Cupriavidus necator
additional information
no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
204500
-
gel filtration
Cupriavidus necator
206000
-
sucrose density gradient centrifugation
Cupriavidus necator
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
; formerly Alcaligenes eutrophus
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxidized hydrogenase purified under aerobic conditions is highly stable but not reactive, reductive activation of the enzyme by H2 in the presence of catalytic amounts of NADH or by reducing agents destabilizes
Cupriavidus necator
Purification (Commentary)
Commentary
Organism
sp. H16, cetavlon, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxyapatite
Cupriavidus necator
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54.46
-
-
Cupriavidus necator
Storage Stability
Storage Stability
Organism
-20°C, O2, several months, no loss in activity
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferricyanide + NAD(P)H
-
439699
Cupriavidus necator
2 ferrocyanide + NAD(P)+ + H+
-
439699
Cupriavidus necator
?
H2 + NAD+
electron acceptor FMN
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor FAD
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor benzyl viologen
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor methyl viologen
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
weak electron acceptor O2
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
enzyme also has diaphorase and NAD(P)H oxidase activity
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor NAD+
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor ferricyanide
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor methylene blue
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor FMN
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + NAD+
electron acceptor FAD
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + NAD+
artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + oxidized benzyl viologen
-
439699
Cupriavidus necator
H+ + reduced benzyl viologen
-
439699
Cupriavidus necator
r
H2 + oxidized methyl viologen
-
439699
Cupriavidus necator
H+ + reduced methyl viologen
-
439699
Cupriavidus necator
r
H2 + oxidized methyl viologen
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + reduced methyl viologen
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator
NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator
?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
?
oxidized cytochrome c + NAD(P)H
-
439699
Cupriavidus necator
reduced cytchrome c + NAD(P)+
-
439699
Cupriavidus necator
?
oxidized methylene blue + NAD(P)H
-
439699
Cupriavidus necator
reduced methylene blue + NAD(P)+
-
439699
Cupriavidus necator
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
33
-
-
Cupriavidus necator
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Cupriavidus necator
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
30% stimulation
Cupriavidus necator
flavin
flavoprotein
Cupriavidus necator
FMN
25% stimulation
Cupriavidus necator
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
30% stimulation
Cupriavidus necator
flavin
flavoprotein
Cupriavidus necator
FMN
25% stimulation
Cupriavidus necator
General Stability (protein specific)
General Stability
Organism
unstable in reduced active form
Cupriavidus necator
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
high ionic strength
200 mM phosphate buffer, 70% inhibition
Cupriavidus necator
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
H2
-
Cupriavidus necator
0.123
-
NAD+
-
Cupriavidus necator
0.56
-
NAD+
-
Cupriavidus necator
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Cupriavidus necator
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+; non-heme iron protein
Cupriavidus necator
additional information
no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
204500
-
gel filtration
Cupriavidus necator
206000
-
sucrose density gradient centrifugation
Cupriavidus necator
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxidized hydrogenase purified under aerobic conditions is highly stable but not reactive, reductive activation of the enzyme by H2 in the presence of catalytic amounts of NADH or by reducing agents destabilizes
Cupriavidus necator
Purification (Commentary) (protein specific)
Commentary
Organism
sp. H16, cetavlon, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxyapatite
Cupriavidus necator
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54.46
-
-
Cupriavidus necator
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, O2, several months, no loss in activity
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferricyanide + NAD(P)H
-
439699
Cupriavidus necator
2 ferrocyanide + NAD(P)+ + H+
-
439699
Cupriavidus necator
?
H2 + NAD+
electron acceptor FMN
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor FAD
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor benzyl viologen
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor methyl viologen
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
weak electron acceptor O2
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
enzyme also has diaphorase and NAD(P)H oxidase activity
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor NAD+
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor ferricyanide
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
artificial electron acceptor methylene blue
439699
Cupriavidus necator
H+ + NADH
-
439699
Cupriavidus necator
r
H2 + NAD+
electron acceptor FMN
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + NAD+
electron acceptor FAD
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + NAD+
artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + NADH
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
H2 + oxidized benzyl viologen
-
439699
Cupriavidus necator
H+ + reduced benzyl viologen
-
439699
Cupriavidus necator
r
H2 + oxidized methyl viologen
-
439699
Cupriavidus necator
H+ + reduced methyl viologen
-
439699
Cupriavidus necator
r
H2 + oxidized methyl viologen
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
H+ + reduced methyl viologen
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
r
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator
NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator
?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
439699
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
?
oxidized cytochrome c + NAD(P)H
-
439699
Cupriavidus necator
reduced cytchrome c + NAD(P)+
-
439699
Cupriavidus necator
?
oxidized methylene blue + NAD(P)H
-
439699
Cupriavidus necator
reduced methylene blue + NAD(P)+
-
439699
Cupriavidus necator
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
33
-
-
Cupriavidus necator
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Cupriavidus necator
Other publictions for EC 1.12.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742575
Puggioni
Distribution of hydrogenases ...
Cupriavidus necator, Cupriavidus necator ATCC 17699
Front. Genet.
7
223
2016
-
-
-
-
-
-
-
-
-
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2
-
2
-
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2
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2
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2
-
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-
-
-
-
-
-
-
-
-
-
-
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-
743797
De Rosa
[NiFe]-hydrogenase is essenti ...
Synechocystis sp. PCC 6803
Sci. Rep.
5
12424
2015
-
-
-
-
-
-
-
-
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1
-
4
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1
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1
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1
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1
1
-
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-
742373
Burroughs
Solar powered biohydrogen pro ...
Synechocystis sp. PCC 6803
Energy Environ. Sci.
7
3791-3800
2014
-
-
-
-
-
-
-
-
1
-
-
1
-
4
-
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1
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1
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1
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1
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-
-
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-
742695
Cano
-
Improved oxygen tolerance of ...
Synechocystis sp.
Int. J. Hydrogen Energy
39
16872-16884
2014
-
-
-
-
8
-
-
-
-
-
-
1
-
1
-
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1
1
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8
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1
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1
1
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-
-
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-
742858
Gutekunst
The bidirectional NiFe-hydrog ...
Synechocystis sp. PCC 6803
J. Biol. Chem.
289
1930-1937
2014
-
-
-
-
-
-
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-
1
-
4
-
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3
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1
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3
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-
-
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-
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-
725748
Herr
-
Stability enhancement of an O2 ...
Cupriavidus necator, Cupriavidus necator DSM 428
J. Mol. Catal. B
97
169-174
2013
-
1
1
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3
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1
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1
1
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1
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726344
Schiffels
An innovative cloning platform ...
Cupriavidus necator
PLoS ONE
8
e68812
2013
-
1
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-
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7
-
-
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-
1
-
1
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1
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1
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1
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-
-
-
-
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-
712972
Worm
Growth- and substrate-dependen ...
Methanospirillum hungatei, Methanospirillum hungatei JF-1 (DSM 864), Syntrophobacter fumaroxidans, Syntrophobacter fumaroxidans MPOB (DSM 10017)
Microbiology
157
280-289
2011
-
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4
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4
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4
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2
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2
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4
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4
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724156
Ratzka
-
Systematic evaluation of the d ...
Cupriavidus necator, Cupriavidus necator DSM 428
Biocatal. Biotransform.
29
246-252
2011
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1
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3
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Transcriptional regulation of ...
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Purification, crystallization ...
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An AbrB-like protein regulates ...
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Structure of [NiFe] hydrogenas ...
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689959
Fdez Galvan
A QM/MM study of proton transp ...
Desulfovibrio fructosivorans
Proteins
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Ohki
Thiolate-bridged dinuclear iro ...
synthetic construct
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Arai
Crystallization and preliminar ...
Thermococcus kodakarensis
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Rakhely
The role of Hox hydrogenase in ...
Thiocapsa roseopersicina, Thiocapsa roseopersicina Bbs
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688938
Watanabe
Crystal structures of [NiFe] h ...
Thermococcus kodakarensis
Mol. Cell
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2007
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Loescher
Bias from H2 cleavage to produ ...
Cupriavidus necator
Biochemistry
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672282
Serebryakova
Characterization of catalytic ...
Gloeocapsa alpicola, Gloeocapsa alpicola CALU 743
Biochemistry
71
1370-1376
2006
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674924
van der Linden
An improved purification proce ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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247-260
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Loescher
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655307
Leul
Biodiversity of hydrogenases i ...
Frankia sp.
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655890
Burgdorf
The soluble NAD+-Reducing [NiF ...
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672279
Tikhonova
Use of a reverse micelle syste ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochemistry
70
645-651
2005
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Vignais
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H/D exchange reactions and mec ...
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1677-1690
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674165
Burgdorf
Structural and oxidation-state ...
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576-592
2005
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1
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675493
Burgdorf
[NiFe]-hydrogenases of Ralston ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
J. Mol. Microbiol. Biotechnol.
10
181-196
2005
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654285
Rakhely
Cyanobacterial-type, heteropen ...
Thiocapsa roseopersicina
Appl. Environ. Microbiol.
70
722-728
2004
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655638
Mohapatra
A hydrogen-evolving enzyme is ...
Frankia sp. R43
FEMS Microbiol. Lett.
236
235-240
2004
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656298
Van der Linden
The soluble [NiFe]-hydrogenase ...
Cupriavidus necator
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616-626
2004
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656734
Soboh
A multisubunit membrane-bound ...
Caldanaerobacter subterraneus subsp. tengcongensis
Microbiology
150
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2004
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654782
Tikhonova
Chemical modification of catal ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochemistry
68
994-1001
2003
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Burgdorf
Functional analysis by site-di ...
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2002
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-
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439713
Porthun
Expression of a functional NAD ...
Rhodococcus opacus, Rhodococcus opacus MR11
Arch. Microbiol.
177
159-166
2002
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6
1
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654388
Hanczar
Detection and localization of ...
Methylococcus capsulatus
Arch. Microbiol.
177
167-172
2002
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9
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5
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1
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1
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654821
Schmitz
HoxE - a subunit specific for ...
Synechocystis sp.
Biochim. Biophys. Acta
1554
66-74
2002
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1
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2
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4
2
1
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1
-
1
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439712
Happe
Unusual FTIR and EPR propertie ...
Cupriavidus necator
FEBS Lett.
466
259-263
2000
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1
1
4
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3
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1
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439711
Grzeszik
Location, catalytic activity, ...
Achromobacter denitrificans 4a-2, Achromobacter denitrificans, Achromobacter ruhlandii, Cupriavidus necator, Cupriavidus necator Cd2/01, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Rhodococcus opacus
Arch. Microbiol.
167
172-176
1997
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52
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5
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36
3
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5
-
36
3
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744926
Zorin
-
Hydrogenase from the thermoph ...
Thermococcus stetteri, Thermococcus stetteri K-15
FEMS Microbiol. Lett.
142
71-76
1996
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2
1
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1
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2
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1
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1
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439692
Tran-Betcke
Cloning and nucleotide sequenc ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
J. Bacteriol.
172
2920-2929
1990
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1
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4
2
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43
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1
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1
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2
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4
1
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439704
Petrov
Redox-dependent inactivation o ...
Cupriavidus necator, Cupriavidus necator Z1
Arch. Biochem. Biophys.
268
298-305
1989
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6
2
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2
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-
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439705
Petrov
Effect of redox potential on t ...
Cupriavidus necator, Cupriavidus necator Z1
Arch. Biochem. Biophys.
268
287-297
1989
1
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1
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6
1
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1
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2
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439695
Hyman
Reversible and irreversible ef ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochem. J.
254
469-475
1988
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1
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1
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42
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2
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1
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1
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2
-
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439696
Hyman
Role of hydrogen in the activa ...
Cupriavidus necator
Biochem. J.
254
463-468
1988
2
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1
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439694
Rohde
-
Immunocytochemical localizatio ...
Cupriavidus necator
FEMS Microbiol. Lett.
36
83-86
1986
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2
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1
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2
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439697
Popov
-
Inactivation of the NAD-depend ...
Cupriavidus necator, Cupriavidus necator Z1
Biochim. Biophys. Acta
831
297-301
1985
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1
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5
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2
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1
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439698
Popov
-
NAD-dependent hydrogenase from ...
Cupriavidus necator, Cupriavidus necator Z1
Biochim. Biophys. Acta
827
466-471
1985
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12
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12
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1
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2
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439702
Schneider
-
Effect of nickel on activity a ...
Rhodococcus opacus, Rhodococcus opacus 1B
Eur. J. Biochem.
138
553-541
1984
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-
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1
1
6
1
5
6
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4
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1
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1
14
1
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1
3
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1
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1
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6
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1
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1
1
14
1
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-
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3
1
3
-
-
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-
-
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439703
Schneider
Content and localization of FM ...
Rhodococcus opacus 1B, Rhodococcus opacus
Eur. J. Biochem.
142
75-84
1984
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2
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4
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2
1
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439706
Friedrich
-
Nickel and iron incoorporation ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Arch. Microbiol.
140
206-211
1984
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1
2
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40
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2
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1
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2
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-
2
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1
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-
-
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439707
Friedrich
Nickel in the catalytically ac ...
Cupriavidus necator
J. Bacteriol.
152
42-48
1982
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1
1
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2
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1
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1
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-
1
-
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-
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-
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-
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439708
Schneider
Production of superoxide radic ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochem. J.
193
99-107
1981
-
-
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1
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1
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42
1
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2
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1
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1
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1
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2
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-
-
-
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-
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439709
Schneider
The iron-sulphur centres of so ...
Cupriavidus necator
Biochim. Biophys. Acta
578
445-461
1979
-
-
-
-
-
-
6
-
1
1
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3
-
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1
-
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-
1
1
1
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6
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1
1
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1
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1
1
1
-
-
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-
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-
-
-
-
-
-
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439710
Schneider
Identification and quantitativ ...
Cupriavidus necator
Biochem. Biophys. Res. Commun.
84
564-571
1978
-
-
-
-
-
-
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1
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2
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1
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2
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
439700
Schneider
Localization and stability of ...
Cupriavidus necator, Cupriavidus necator b19, Cupriavidus necator G27, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Cupriavidus necator N9A, no activity in Alcaligenes paradoxus, no activity in Aquaspirillum autotrophicum, no activity in Corynebacterium autotrophicum, no activity in Hydrogenophaga palleronii, no activity in Paracoccus denitrificans, no activity in Pseudomonas facilis
Arch. Microbiol.
112
229-238
1977
-
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-
-
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1
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-
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49
-
-
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-
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5
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-
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1
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-
-
5
-
-
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-
-
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-
-
-
-
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-
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-
439699
Schneider
Purification and properties of ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochim. Biophys. Acta
452
66-80
1976
-
-
-
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-
1
1
3
1
2
2
-
-
41
1
-
1
-
-
-
1
1
21
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
1
-
1
-
3
1
2
2
-
-
1
-
1
-
-
1
1
21
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439701
Aggag
Studies on a gram-positive hyd ...
Rhodococcus opacus 1B, Rhodococcus opacus
Arch. Microbiol.
100
25-39
1974
4
-
-
-
-
2
8
2
-
3
-
-
-
6
-
-
1
-
-
-
1
1
10
-
-
-
1
-
2
1
1
1
-
-
-
4
-
-
1
-
-
2
-
8
-
2
-
3
-
-
-
-
-
1
-
-
1
1
10
-
-
-
1
-
2
1
1
-
-
-
-
-
-
-
439693
Pfitzner
Properties of the NAD-specific ...
Hydrogenomonas sp., Hydrogenomonas sp. H16
Arch. Mikrobiol.
71
67-78
1970
-
-
-
-
-
-
2
2
1
3
-
-
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3
-
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1
-
-
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1
1
6
-
1
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-
-
2
1
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-
-
-
-
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-
2
-
2
1
3
-
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1
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1
1
6
-
1
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2
1
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-
-