General Stability | Organism |
---|---|
unstable in reduced active form | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
high ionic strength | 200 mM phosphate buffer, 70% inhibition | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
H2 | - |
Cupriavidus necator | |
0.123 | - |
NAD+ | - |
Cupriavidus necator | |
0.56 | - |
NAD+ | - |
Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Cupriavidus necator | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron protein | Cupriavidus necator | |
Iron | no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+ | Cupriavidus necator | |
additional information | no increase of activity by addition of Co2+, Mn2+, Ni2+ or Fe2+ | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
204500 | - |
gel filtration | Cupriavidus necator |
206000 | - |
sucrose density gradient centrifugation | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator | - |
formerly Alcaligenes eutrophus | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxidized hydrogenase purified under aerobic conditions is highly stable but not reactive, reductive activation of the enzyme by H2 in the presence of catalytic amounts of NADH or by reducing agents destabilizes | Cupriavidus necator |
Purification (Comment) | Organism |
---|---|
sp. H16, cetavlon, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxyapatite | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.46 | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
-20°C, O2, several months, no loss in activity | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NAD(P)H | - |
Cupriavidus necator | 2 ferrocyanide + NAD(P)+ + H+ | - |
? | |
H2 + NAD+ | electron acceptor FMN | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | electron acceptor FAD | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptor benzyl viologen | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptor methyl viologen | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | weak electron acceptor O2 | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | enzyme also has diaphorase and NAD(P)H oxidase activity | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | electron acceptor NAD+ | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptor ferricyanide | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptor methylene blue | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | electron acceptor FMN | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + NADH | - |
r | |
H2 + NAD+ | electron acceptor FAD | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + NADH | - |
r | |
H2 + NAD+ | artificial electron acceptors: Janus green, 2,6-dichlorophenolindophenol, phenazine methosulfate, menaquinone, ubiquinone, cytochrome c | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + NADH | - |
r | |
H2 + oxidized benzyl viologen | - |
Cupriavidus necator | H+ + reduced benzyl viologen | - |
r | |
H2 + oxidized methyl viologen | - |
Cupriavidus necator | H+ + reduced methyl viologen | - |
r | |
H2 + oxidized methyl viologen | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + reduced methyl viologen | - |
r | |
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Cupriavidus necator | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
oxidized cytochrome c + NAD(P)H | - |
Cupriavidus necator | reduced cytchrome c + NAD(P)+ | - |
? | |
oxidized methylene blue + NAD(P)H | - |
Cupriavidus necator | reduced methylene blue + NAD(P)+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | 30% stimulation | Cupriavidus necator | |
flavin | flavoprotein | Cupriavidus necator | |
FMN | 25% stimulation | Cupriavidus necator |