BRENDA - Enzyme Database
show all sequences of 1.11.2.6

A heme peroxidase with a functional role as an L-tyrosine hydroxylase in the biosynthesis of anthramycin

Connor, K.L.; Colabroy, K.L.; Gerratana, B.; Biochemistry 50, 8926-8936 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces refuineus
Inhibitors
Inhibitors
Commentary
Organism
Structure
H2O2
the enzyme becomes inactivated by hydrogen peroxide during catalysis
Streptomyces refuineus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.45
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
1
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-tyrosine + H2O2
Streptomyces refuineus
-
L-dopa + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces refuineus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-Sepharose column chromatography or Q-Sepharose column chromatography and S-200HR gel filtration
Streptomyces refuineus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-(4-hydroxylphenyl)propanoic acid + H2O2
-
756033
Streptomyces refuineus
3-(3,4-hydroxyphenyl) propanoic acid + H2O
-
-
-
?
4-cresol + H2O2
-
756033
Streptomyces refuineus
4-methylcatechol + H2O
-
-
-
?
DL-tyrosine + H2O2
-
756033
Streptomyces refuineus
L-DOPA + H2O
-
-
-
?
L-tyrosine + H2O2
-
756033
Streptomyces refuineus
L-dopa + H2O
-
-
-
?
additional information
no activity with L-phenylalanine
756033
Streptomyces refuineus
?
-
-
-
-
tyramine + H2O2
-
756033
Streptomyces refuineus
dopamine + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33600, SDS-PAGE
Streptomyces refuineus
monomer
1 * 37900, calculated from amino acid sequence
Streptomyces refuineus
Synonyms
Synonyms
Commentary
Organism
Orf13
-
Streptomyces refuineus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.576
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
1.5
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
Cofactor
Cofactor
Commentary
Organism
Structure
heme b
required for catalysis
Streptomyces refuineus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces refuineus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme b
required for catalysis
Streptomyces refuineus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
H2O2
the enzyme becomes inactivated by hydrogen peroxide during catalysis
Streptomyces refuineus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.45
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
1
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-tyrosine + H2O2
Streptomyces refuineus
-
L-dopa + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-Sepharose column chromatography or Q-Sepharose column chromatography and S-200HR gel filtration
Streptomyces refuineus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-(4-hydroxylphenyl)propanoic acid + H2O2
-
756033
Streptomyces refuineus
3-(3,4-hydroxyphenyl) propanoic acid + H2O
-
-
-
?
4-cresol + H2O2
-
756033
Streptomyces refuineus
4-methylcatechol + H2O
-
-
-
?
DL-tyrosine + H2O2
-
756033
Streptomyces refuineus
L-DOPA + H2O
-
-
-
?
L-tyrosine + H2O2
-
756033
Streptomyces refuineus
L-dopa + H2O
-
-
-
?
additional information
no activity with L-phenylalanine
756033
Streptomyces refuineus
?
-
-
-
-
tyramine + H2O2
-
756033
Streptomyces refuineus
dopamine + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33600, SDS-PAGE
Streptomyces refuineus
monomer
1 * 37900, calculated from amino acid sequence
Streptomyces refuineus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.576
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
1.5
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.012
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
0.015
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.012
-
L-tyrosine
at pH 8.0 and 37°C
Streptomyces refuineus
0.015
-
H2O2
with L-tyrosine as cosubstrate, at pH 8.0 and 37°C
Streptomyces refuineus
Other publictions for EC 1.11.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755672
Wang
Biocatalytic carbon-hydrogen ...
Streptomyces lincolnensis
ACS Catal.
9
4764-4776
2019
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
756033
Connor
A heme peroxidase with a func ...
Streptomyces refuineus
Biochemistry
50
8926-8936
2011
-
-
1
-
-
-
1
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
6
2
1
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
2
-
-
-
1
-
-
-
1
-
-
-
-
6
2
-
-
-
2
-
-
-
-
-
-
-
-
2
2
755910
Neusser
The genes ImbB1 and ImbB2 of ...
Streptomyces lincolnensis
Arch. Microbiol.
169
322-332
1998
-
-
1
-
-
-
1
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-