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Literature summary for 1.11.2.4 extracted from
- Crystallization and preliminary X-ray diffraction analysis of fatty-acid hydroxylase cytochrome P450BSbeta from Bacillus subtilis (2002), Acta Crystallogr. Sect. D, 58, 687-689.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 10% (w/v) PEG 4000 and 50 mM MES (pH 6.8) or 10% (w/v) PEG 4000, 0.15 mM magnesium acetate and 50 mM MES (pH 6.5) in the presence of 2 mM myristic acid | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O31440 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristic acid + H2O2 | - |
Bacillus subtilis | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 * 48000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P450BSbeta | - |
Bacillus subtilis |
| fatty-acid hydroxylase | - |
Bacillus subtilis |
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