BRENDA - Enzyme Database
show all sequences of 1.11.2.3

Epoxidation, hydroxylation and aromatization is catalyzed by a peroxygenase from Solanum lycopersicum

Fuchs, C.; Schwab, W.; J. Mol. Catal. B 96, 52-60 (2013)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cervisiae
Solanum lycopersicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.071
-
oleic acid
pH 6.0, 40°C
Solanum lycopersicum
0.078
-
linoleic acid
pH 6.0, 40°C
Solanum lycopersicum
0.815
-
trans-10,cis-12-linoleic acid
pH 6.0, 40°C
Solanum lycopersicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27900
-
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
28000
-
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-(R)-limonene + H2O2
-
725747
Solanum lycopersicum
(R)-limonene trans-1,2-epoxide
97% of products
-
-
?
(-)-(S)-limonene + H2O2
-
725747
Solanum lycopersicum
(S)-limonene cis-1,2-epoxide
88% of products
-
-
?
alpha-terpinene + H2O2
-
725747
Solanum lycopersicum
cis-1,4-dihydroxy-p-menth-2-ene
-
-
-
?
gamma-terpinene + H2O2
-
725747
Solanum lycopersicum
p-cymene
in this reaction, hydroperoxide serves as hydrogen acceptor rather than an oxygen donor
-
-
?
linoleic acid + H2O2
-
725747
Solanum lycopersicum
15,16-epoxy-9,12-octadecadienoic acid + H2O
25% yield
-
-
?
additional information
no substrate: geraniol
725747
Solanum lycopersicum
?
-
-
-
-
nerol + H2O2
-
725747
Solanum lycopersicum
nerol-2,3-epoxide
sole product
-
-
?
oleic acid + H2O2
-
725747
Solanum lycopersicum
9,10-epoxyoctadecanoic acid
15% yield
-
-
?
trans-10,cis-12-linoleic acid + H2O2
-
725747
Solanum lycopersicum
12,13-epoxy-10-octadecenoic acid
epoxidation occurs only at cis-double bond
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Solanum lycopersicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Solanum lycopersicum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cervisiae
Solanum lycopersicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required
Solanum lycopersicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.071
-
oleic acid
pH 6.0, 40°C
Solanum lycopersicum
0.078
-
linoleic acid
pH 6.0, 40°C
Solanum lycopersicum
0.815
-
trans-10,cis-12-linoleic acid
pH 6.0, 40°C
Solanum lycopersicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27900
-
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
28000
-
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-(R)-limonene + H2O2
-
725747
Solanum lycopersicum
(R)-limonene trans-1,2-epoxide
97% of products
-
-
?
(-)-(S)-limonene + H2O2
-
725747
Solanum lycopersicum
(S)-limonene cis-1,2-epoxide
88% of products
-
-
?
alpha-terpinene + H2O2
-
725747
Solanum lycopersicum
cis-1,4-dihydroxy-p-menth-2-ene
-
-
-
?
gamma-terpinene + H2O2
-
725747
Solanum lycopersicum
p-cymene
in this reaction, hydroperoxide serves as hydrogen acceptor rather than an oxygen donor
-
-
?
linoleic acid + H2O2
-
725747
Solanum lycopersicum
15,16-epoxy-9,12-octadecadienoic acid + H2O
25% yield
-
-
?
additional information
no substrate: geraniol
725747
Solanum lycopersicum
?
-
-
-
-
nerol + H2O2
-
725747
Solanum lycopersicum
nerol-2,3-epoxide
sole product
-
-
?
oleic acid + H2O2
-
725747
Solanum lycopersicum
9,10-epoxyoctadecanoic acid
15% yield
-
-
?
trans-10,cis-12-linoleic acid + H2O2
-
725747
Solanum lycopersicum
12,13-epoxy-10-octadecenoic acid
epoxidation occurs only at cis-double bond
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27900, calculated, x * 28000, SDS-PAGE
Solanum lycopersicum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Solanum lycopersicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Solanum lycopersicum
Other publictions for EC 1.11.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742334
Shimada
Leaf oil bodies are subcellul ...
Arabidopsis thaliana
Curr. Opin. Plant Biol.
25
145-150
2015
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-
-
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-
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1
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3
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1
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1
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3
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1
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1
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1
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3
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1
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3
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1
1
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-
743541
Hanano
Involvement of the caleosin/p ...
Arabidopsis thaliana
Plant Signal. Behav.
10
e991574
2015
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6
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1
1
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743492
Blee
The reductase activity of the ...
Arabidopsis thaliana
Plant Physiol.
166
109-124
2014
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1
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4
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1
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11
1
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1
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1
1
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1
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11
1
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1
1
-
-
-
725747
Fuchs
-
Epoxidation, hydroxylation and ...
Solanum lycopersicum
J. Mol. Catal. B
96
52-60
2013
-
-
1
-
-
-
-
3
-
-
2
-
-
1
-
-
-
-
-
-
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-
9
1
1
-
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-
1
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1
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1
1
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3
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2
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9
1
1
-
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-
1
-
-
-
-
-
-
-
-
-
724973
Blee
A non-canonical caleosin from ...
Arabidopsis thaliana
FEBS J.
279
3981-3995
2012
-
-
1
-
-
-
-
-
1
1
1
3
-
4
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
-
-
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1
1
1
3
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-
4
-
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3
1
-
-
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-
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-
726143
Kim
A stress-responsive caleosin-l ...
Arabidopsis thaliana
Plant Cell Physiol.
52
874-884
2011
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1
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3
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2
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1
1
1
1
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-
726222
Meesapyodsuk
A peroxygenase pathway involve ...
Avena sativa
Plant Physiol.
157
454-463
2011
-
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1
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2
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1
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6
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1
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1
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1
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-
6
-
1
-
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-
1
1
-
-
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-
-
-
-
-
713295
Partridge
Roles of a membrane-bound cale ...
Arabidopsis thaliana
Plant Physiol. Biochem.
47
796-806
2009
-
-
-
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-
-
-
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3
1
2
1
-
4
-
1
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6
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1
1
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3
1
2
1
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1
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6
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1
1
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1
1
1
1
-
-
712256
Piazza
-
One-pot synthesis of fatty aci ...
Avena sativa
J. Am. Oil Chem. Soc.
83
1021-1025
2006
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1
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4
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1
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4
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1
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1
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4
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1
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4
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1
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712359
Hanano
Plant seed peroxygenase is an ...
Arabidopsis thaliana, Avena sativa
J. Biol. Chem.
281
33140-33151
2006
-
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2
-
2
-
2
-
4
2
2
10
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7
-
3
2
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3
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10
-
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2
-
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2
2
-
2
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-
2
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4
2
2
10
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3
2
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3
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10
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671684
Magri
-
Immobilization of soybean seed ...
Glycine max
Biocatal. Biotransform.
23
339-346
2005
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1
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1
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1
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1
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2
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712357
Kandel
Cloning, functional expression ...
Triticum aestivum
J. Biol. Chem.
280
35881-35889
2005
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1
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712255
Piazza
-
Synthesis of a fatty tetrahydr ...
Avena sativa
J. Am. Oil Chem. Soc.
81
933-937
2004
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1
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1
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1
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712776
Piazza
-
Epoxidation of fatty acids, fa ...
Avena sativa
J. Mol. Catal. B
21
143-151
2003
-
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1
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1
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1
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15
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1
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1
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1
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1
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15
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713275
Lequeu
Formation of plant cuticle: ev ...
Avena sativa, Glycine max, Zea mays
Plant J.
36
155-164
2003
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18
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3
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6
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3
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15
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15
18
3
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1
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3
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6
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6
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439735
Henriksen
Structure of soybean seed coat ...
Soja hispida
Protein Sci.
10
108-115
2001
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1
1
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1
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1
1
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1
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1
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711277
Takamura
Oxygenation of (3Z)-alkenal to ...
Glycine max
Biochim. Biophys. Acta
1303
83-91
1996
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4
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1
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713303
Hamberg
Peroxygenase-catalyzed fatty a ...
Avena sativa, Hordeum vulgare, Hordeum vulgare subsp. vulgare, Secale cereale, Triticum aestivum
Plant Physiol.
110
807-815
1996
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-
-
-
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-
-
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6
-
-
-
-
5
-
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5
-
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25
-
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5
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5
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6
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5
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25
-
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711060
Doerge
Oxidation of organosulfur comp ...
Pisum sativum
Biochem. Biophys. Res. Commun.
121
1001-1005
1984
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1
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1
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712329
Ishimaru
Purification and characterizat ...
Pisum sativum
J. Biol. Chem.
254
8427-8433
1979
-
-
-
-
-
-
-
4
2
-
3
1
3
1
-
-
1
-
-
1
2
-
3
1
-
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
2
-
3
1
3
-
-
1
-
1
2
-
3
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-