Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with membrane | Arabidopsis thaliana | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + cumene hydroperoxide | Arabidopsis thaliana | - |
? | 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 | ? | |
linolenic acid + cumene hydroperoxide | Arabidopsis thaliana | 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 | linolenic acid 9,10-15,16-diepoxide + ? | major product, (R),(S)-epoxide enantiomers | ? | |
additional information | Arabidopsis thaliana | enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9CAB7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | expression in root, stem, leaf and flower | Arabidopsis thaliana | - |
leaf | expression in root, stem, leaf and flower | Arabidopsis thaliana | - |
root | expression in root, stem, leaf and flower | Arabidopsis thaliana | - |
stem | expression in root, stem, leaf and flower | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + cumene hydroperoxide | - |
Arabidopsis thaliana | ? | 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 | ? | |
linolenic acid + cumene hydroperoxide | 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 | Arabidopsis thaliana | linolenic acid 9,10-15,16-diepoxide + ? | major product, (R),(S)-epoxide enantiomers | ? | |
additional information | enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
PXG4 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | contains a protoheme of type b, with 0.6 nmol of heme per mg protein | Arabidopsis thaliana |