BRENDA - Enzyme Database show
show all sequences of 1.11.2.3

A non-canonical caleosin from Arabidopsis efficiently epoxidizes physiological unsaturated fatty acids with complete stereoselectivity

Blee, E.; Flenet, M.; Boachon, B.; Fauconnier, M.L.; FEBS J. 279, 3981-3995 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated with membrane
Arabidopsis thaliana
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
x * 24000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
?
linolenic acid + cumene hydroperoxide
Arabidopsis thaliana
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
?
additional information
Arabidopsis thaliana
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9CAB7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
leaf
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
root
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
stem
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleic acid + cumene hydroperoxide
-
724973
Arabidopsis thaliana
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
-
?
linolenic acid + cumene hydroperoxide
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
724973
Arabidopsis thaliana
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
-
?
additional information
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
724973
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 24000, SDS-PAGE
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
heme
contains a protoheme of type b, with 0.6 nmol of heme per mg protein
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
contains a protoheme of type b, with 0.6 nmol of heme per mg protein
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated with membrane
Arabidopsis thaliana
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
x * 24000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
?
linolenic acid + cumene hydroperoxide
Arabidopsis thaliana
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
?
additional information
Arabidopsis thaliana
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
leaf
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
root
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
stem
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linoleic acid + cumene hydroperoxide
-
724973
Arabidopsis thaliana
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
-
?
linolenic acid + cumene hydroperoxide
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
724973
Arabidopsis thaliana
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
-
?
additional information
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
724973
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 24000, SDS-PAGE
Arabidopsis thaliana
Other publictions for EC 1.11.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742334
Shimada
Leaf oil bodies are subcellul ...
Arabidopsis thaliana
Curr. Opin. Plant Biol.
25
145-150
2015
-
-
-
-
-
-
-
-
1
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3
-
1
-
-
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1
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3
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1
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1
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1
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3
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1
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-
3
-
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-
-
-
-
-
-
1
1
-
-
-
743541
Hanano
Involvement of the caleosin/p ...
Arabidopsis thaliana
Plant Signal. Behav.
10
e991574
2015
-
-
-
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6
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1
1
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743492
Blee
The reductase activity of the ...
Arabidopsis thaliana
Plant Physiol.
166
109-124
2014
-
-
1
-
-
-
-
-
-
-
-
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-
4
-
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1
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11
1
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1
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1
1
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1
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11
1
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-
1
1
-
-
-
725747
Fuchs
-
Epoxidation, hydroxylation and ...
Solanum lycopersicum
J. Mol. Catal. B
96
52-60
2013
-
-
1
-
-
-
-
3
-
-
2
-
-
1
-
-
-
-
-
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9
1
1
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1
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1
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1
1
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-
-
-
-
-
3
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2
-
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-
-
-
-
-
-
-
9
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724973
Blee
A non-canonical caleosin from ...
Arabidopsis thaliana
FEBS J.
279
3981-3995
2012
-
-
1
-
-
-
-
-
1
1
1
3
-
4
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
1
1
3
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726143
Kim
A stress-responsive caleosin-l ...
Arabidopsis thaliana
Plant Cell Physiol.
52
874-884
2011
-
-
1
-
-
-
-
-
-
-
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3
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2
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1
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-
2
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-
-
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
726222
Meesapyodsuk
A peroxygenase pathway involve ...
Avena sativa
Plant Physiol.
157
454-463
2011
-
-
1
-
-
-
-
-
2
-
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1
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6
-
1
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1
1
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1
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2
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6
-
1
-
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-
1
1
-
-
-
-
-
-
-
-
713295
Partridge
Roles of a membrane-bound cale ...
Arabidopsis thaliana
Plant Physiol. Biochem.
47
796-806
2009
-
-
-
-
-
-
-
-
3
1
2
1
-
4
-
1
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-
6
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1
1
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-
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-
-
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-
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-
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-
-
-
-
3
1
2
1
-
-
1
-
-
6
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712256
Piazza
One-pot synthesis of fatty aci ...
Avena sativa
J. Am. Oil Chem. Soc.
83
1021-1025
2006
-
-
-
-
-
-
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1
-
4
-
1
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1
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4
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1
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1
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4
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1
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4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
712359
Hanano
Plant seed peroxygenase is an ...
Arabidopsis thaliana, Avena sativa
J. Biol. Chem.
281
33140-33151
2006
-
-
2
-
2
-
2
-
4
2
2
10
-
7
-
3
2
-
-
3
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
2
-
-
2
-
-
4
2
2
10
-
-
3
2
-
3
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671684
Magri
-
Immobilization of soybean seed ...
Glycine max
Biocatal. Biotransform.
23
339-346
2005
-
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-
1
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1
1
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1
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1
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2
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1
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1
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1
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1
-
2
-
-
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-
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-
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-
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-
712357
Kandel
Cloning, functional expression ...
Triticum aestivum
J. Biol. Chem.
280
35881-35889
2005
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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1
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1
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712255
Piazza
Synthesis of a fatty tetrahydr ...
Avena sativa
J. Am. Oil Chem. Soc.
81
933-937
2004
-
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1
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1
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1
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1
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1
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-
712776
Piazza
-
Epoxidation of fatty acids, fa ...
Avena sativa
J. Mol. Catal. B
21
143-151
2003
-
-
-
-
-
-
-
-
1
-
-
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-
1
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1
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-
15
-
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1
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1
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1
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1
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-
15
-
-
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713275
Lequeu
Formation of plant cuticle: ev ...
Avena sativa, Glycine max, Zea mays
Plant J.
36
155-164
2003
-
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18
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1
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3
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3
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6
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3
-
15
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15
18
3
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1
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3
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6
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6
6
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439735
Henriksen
Structure of soybean seed coat ...
Soja hispida
Protein Sci.
10
108-115
2001
-
-
1
1
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-
2
1
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1
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1
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1
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1
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1
1
1
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2
1
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1
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1
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711277
Takamura
Oxygenation of (3Z)-alkenal to ...
Glycine max
Biochim. Biophys. Acta
1303
83-91
1996
-
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1
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1
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3
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1
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4
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1
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1
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1
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4
-
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1
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713303
Hamberg
Peroxygenase-catalyzed fatty a ...
Avena sativa, Hordeum vulgare, Hordeum vulgare subsp. vulgare, Secale cereale, Triticum aestivum
Plant Physiol.
110
807-815
1996
-
-
-
-
-
-
-
-
6
-
-
-
-
5
-
-
-
-
-
5
-
-
25
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
6
-
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-
-
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5
-
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25
-
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-
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711060
Doerge
Oxidation of organosulfur comp ...
Pisum sativum
Biochem. Biophys. Res. Commun.
121
1001-1005
1984
-
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3
1
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1
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1
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4
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1
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1
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3
1
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1
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4
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712329
Ishimaru
Purification and characterizat ...
Pisum sativum
J. Biol. Chem.
254
8427-8433
1979
-
-
-
-
-
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-
4
2
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3
1
3
1
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1
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1
2
-
3
1
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3
-
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1
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-
1
-
-
-
-
-
-
4
2
-
3
1
3
-
-
1
-
1
2
-
3
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-