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Literature summary for 1.11.2.3 extracted from

  • Blee, E.; Flenet, M.; Boachon, B.; Fauconnier, M.L.
    A non-canonical caleosin from Arabidopsis efficiently epoxidizes physiological unsaturated fatty acids with complete stereoselectivity (2012), FEBS J., 279, 3981-3995.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with membrane Arabidopsis thaliana 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleic acid + cumene hydroperoxide Arabidopsis thaliana
-
? 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 ?
linolenic acid + cumene hydroperoxide Arabidopsis thaliana 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 linolenic acid 9,10-15,16-diepoxide + ? major product, (R),(S)-epoxide enantiomers ?
additional information Arabidopsis thaliana enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9CAB7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower expression in root, stem, leaf and flower Arabidopsis thaliana
-
leaf expression in root, stem, leaf and flower Arabidopsis thaliana
-
root expression in root, stem, leaf and flower Arabidopsis thaliana
-
stem expression in root, stem, leaf and flower Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + cumene hydroperoxide
-
Arabidopsis thaliana ? 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 ?
linolenic acid + cumene hydroperoxide 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 Arabidopsis thaliana linolenic acid 9,10-15,16-diepoxide + ? major product, (R),(S)-epoxide enantiomers ?
additional information enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
PXG4
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
heme contains a protoheme of type b, with 0.6 nmol of heme per mg protein Arabidopsis thaliana