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Literature summary for 1.11.2.3 extracted from

  • Hanano, A.; Burcklen, M.; Flenet, M.; Ivancich, A.; Louwagie, M.; Garin, J.; Blee, E.
    Plant seed peroxygenase is an original heme-oxygenase with an EF-hand calcium binding motif (2006), J. Biol. Chem., 281, 33140-33151.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Arabidopsis thaliana
expressed in Saccharomyces cerevisiae Avena sativa

Protein Variants

Protein Variants Comment Organism
H138V the mutant possess only 4% of the heme content wild type isoform PXG1 Arabidopsis thaliana
H70V the heme content of the mutant is barely measurable (less than 1%) as compared to the wild type isoform PXG1 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol the sulfoxidation of thiobenzamide, catalyzed by isoform PXG1, is completely abolished in the presence of 1 mM 2-mercaptoethanol (competitive inhibitor) Arabidopsis thaliana
terbufos a suicide substrate for plant peroxygenases, and at a concentration of 3 mM, it effectively inactivates the activity of isoform PXG1 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Arabidopsis thaliana 16020
-
membrane integral membrane protein Avena sativa 16020
-
microsome
-
Arabidopsis thaliana
-
-
microsome
-
Avena sativa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme contains a Ca2+ binding motif, calcium is crucial for its peroxygenase activity Arabidopsis thaliana
Ca2+ the enzyme contains a Ca2+ binding motif, calcium is crucial for peroxygenase activity Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
SDS-PAGE Avena sativa
32000
-
SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aniline + cumene hydroperoxide Arabidopsis thaliana
-
?
-
?
aniline + cumene hydroperoxide Avena sativa
-
?
-
?
linoleic acid + cumene hydroperoxide Arabidopsis thaliana
-
?
-
?
linoleic acid + cumene hydroperoxide Avena sativa
-
?
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide Arabidopsis thaliana
-
?
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide Avena sativa
-
?
-
?
oleic acid + cumene hydroperoxide Arabidopsis thaliana
-
cis-9,10-epoxystearic acid + ?
-
?
oleic acid + cumene hydroperoxide Avena sativa
-
cis-9,10-epoxystearic acid + ?
-
?
thiobenzamide + cumene hydroperoxide Arabidopsis thaliana
-
?
-
?
thiobenzamide + cumene hydroperoxide Avena sativa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Avena sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Arabidopsis thaliana
glycoprotein
-
Avena sativa
sumoylation isoform PXG1 possesses a high sumoylation potentiality at the position Lys-196 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography Arabidopsis thaliana
Ni-NTA column chromatography Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arabidopsis thaliana
-
seed
-
Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aniline + cumene hydroperoxide
-
Arabidopsis thaliana ?
-
?
aniline + cumene hydroperoxide
-
Avena sativa ?
-
?
linoleic acid + cumene hydroperoxide
-
Arabidopsis thaliana ?
-
?
linoleic acid + cumene hydroperoxide
-
Avena sativa ?
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
Arabidopsis thaliana ?
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
Avena sativa ?
-
?
oleic acid + cumene hydroperoxide
-
Arabidopsis thaliana cis-9,10-epoxystearic acid + ?
-
?
oleic acid + cumene hydroperoxide
-
Avena sativa cis-9,10-epoxystearic acid + ?
-
?
thiobenzamide + cumene hydroperoxide
-
Arabidopsis thaliana ?
-
?
thiobenzamide + cumene hydroperoxide
-
Avena sativa ?
-
?

Synonyms

Synonyms Comment Organism
caleosin
-
Arabidopsis thaliana
caleosin
-
Avena sativa
plant peroxygenase
-
Arabidopsis thaliana
plant peroxygenase
-
Avena sativa
PXG
-
Avena sativa
PXG1 isoform Arabidopsis thaliana
PXG2 isoform Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
heme
-
Avena sativa
heme isoform PXG1 is a hemoprotein Arabidopsis thaliana