Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Arabidopsis thaliana |
expressed in Saccharomyces cerevisiae | Avena sativa |
Protein Variants | Comment | Organism |
---|---|---|
H138V | the mutant possess only 4% of the heme content wild type isoform PXG1 | Arabidopsis thaliana |
H70V | the heme content of the mutant is barely measurable (less than 1%) as compared to the wild type isoform PXG1 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | the sulfoxidation of thiobenzamide, catalyzed by isoform PXG1, is completely abolished in the presence of 1 mM 2-mercaptoethanol (competitive inhibitor) | Arabidopsis thaliana | |
terbufos | a suicide substrate for plant peroxygenases, and at a concentration of 3 mM, it effectively inactivates the activity of isoform PXG1 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Arabidopsis thaliana | 16020 | - |
membrane | integral membrane protein | Avena sativa | 16020 | - |
microsome | - |
Arabidopsis thaliana | - |
- |
microsome | - |
Avena sativa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme contains a Ca2+ binding motif, calcium is crucial for its peroxygenase activity | Arabidopsis thaliana | |
Ca2+ | the enzyme contains a Ca2+ binding motif, calcium is crucial for peroxygenase activity | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
SDS-PAGE | Avena sativa |
32000 | - |
SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aniline + cumene hydroperoxide | Arabidopsis thaliana | - |
? | - |
? | |
aniline + cumene hydroperoxide | Avena sativa | - |
? | - |
? | |
linoleic acid + cumene hydroperoxide | Arabidopsis thaliana | - |
? | - |
? | |
linoleic acid + cumene hydroperoxide | Avena sativa | - |
? | - |
? | |
methyl 4-tolyl sulfide + cumene hydroperoxide | Arabidopsis thaliana | - |
? | - |
? | |
methyl 4-tolyl sulfide + cumene hydroperoxide | Avena sativa | - |
? | - |
? | |
oleic acid + cumene hydroperoxide | Arabidopsis thaliana | - |
cis-9,10-epoxystearic acid + ? | - |
? | |
oleic acid + cumene hydroperoxide | Avena sativa | - |
cis-9,10-epoxystearic acid + ? | - |
? | |
thiobenzamide + cumene hydroperoxide | Arabidopsis thaliana | - |
? | - |
? | |
thiobenzamide + cumene hydroperoxide | Avena sativa | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Avena sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Arabidopsis thaliana |
glycoprotein | - |
Avena sativa |
sumoylation | isoform PXG1 possesses a high sumoylation potentiality at the position Lys-196 | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography | Arabidopsis thaliana |
Ni-NTA column chromatography | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arabidopsis thaliana | - |
seed | - |
Avena sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aniline + cumene hydroperoxide | - |
Arabidopsis thaliana | ? | - |
? | |
aniline + cumene hydroperoxide | - |
Avena sativa | ? | - |
? | |
linoleic acid + cumene hydroperoxide | - |
Arabidopsis thaliana | ? | - |
? | |
linoleic acid + cumene hydroperoxide | - |
Avena sativa | ? | - |
? | |
methyl 4-tolyl sulfide + cumene hydroperoxide | - |
Arabidopsis thaliana | ? | - |
? | |
methyl 4-tolyl sulfide + cumene hydroperoxide | - |
Avena sativa | ? | - |
? | |
oleic acid + cumene hydroperoxide | - |
Arabidopsis thaliana | cis-9,10-epoxystearic acid + ? | - |
? | |
oleic acid + cumene hydroperoxide | - |
Avena sativa | cis-9,10-epoxystearic acid + ? | - |
? | |
thiobenzamide + cumene hydroperoxide | - |
Arabidopsis thaliana | ? | - |
? | |
thiobenzamide + cumene hydroperoxide | - |
Avena sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caleosin | - |
Arabidopsis thaliana |
caleosin | - |
Avena sativa |
plant peroxygenase | - |
Arabidopsis thaliana |
plant peroxygenase | - |
Avena sativa |
PXG | - |
Avena sativa |
PXG1 | isoform | Arabidopsis thaliana |
PXG2 | isoform | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Avena sativa | |
heme | isoform PXG1 is a hemoprotein | Arabidopsis thaliana |