BRENDA - Enzyme Database show
show all sequences of 1.11.2.3

Plant seed peroxygenase is an original heme-oxygenase with an EF-hand calcium binding motif

Hanano, A.; Burcklen, M.; Flenet, M.; Ivancich, A.; Louwagie, M.; Garin, J.; Blee, E.; J. Biol. Chem. 281, 33140-33151 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Avena sativa
expressed in Saccharomyces cerevisiae
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
H138V
the mutant possess only 4% of the heme content wild type isoform PXG1
Arabidopsis thaliana
H70V
the heme content of the mutant is barely measurable (less than 1%) as compared to the wild type isoform PXG1
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
the sulfoxidation of thiobenzamide, catalyzed by isoform PXG1, is completely abolished in the presence of 1 mM 2-mercaptoethanol (competitive inhibitor)
Arabidopsis thaliana
terbufos
a suicide substrate for plant peroxygenases, and at a concentration of 3 mM, it effectively inactivates the activity of isoform PXG1
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Arabidopsis thaliana
16020
-
membrane
integral membrane protein
Avena sativa
16020
-
microsome
-
Arabidopsis thaliana
-
-
microsome
-
Avena sativa
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains a Ca2+ binding motif, calcium is crucial for its peroxygenase activity
Arabidopsis thaliana
Ca2+
the enzyme contains a Ca2+ binding motif, calcium is crucial for peroxygenase activity
Avena sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
SDS-PAGE
Avena sativa
32000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aniline + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
aniline + cumene hydroperoxide
Avena sativa
-
?
-
-
?
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
linoleic acid + cumene hydroperoxide
Avena sativa
-
?
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
Avena sativa
-
?
-
-
?
oleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
cis-9,10-epoxystearic acid + ?
-
-
?
oleic acid + cumene hydroperoxide
Avena sativa
-
cis-9,10-epoxystearic acid + ?
-
-
?
thiobenzamide + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
thiobenzamide + cumene hydroperoxide
Avena sativa
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Avena sativa
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Arabidopsis thaliana
glycoprotein
-
Avena sativa
sumoylation
isoform PXG1 possesses a high sumoylation potentiality at the position Lys-196
Arabidopsis thaliana
Purification (Commentary)
Commentary
Organism
DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography
Arabidopsis thaliana
Ni-NTA column chromatography
Avena sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Arabidopsis thaliana
-
seed
-
Avena sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
aniline + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
aniline + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
linoleic acid + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
linoleic acid + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
oleic acid + cumene hydroperoxide
-
712359
Arabidopsis thaliana
cis-9,10-epoxystearic acid + ?
-
-
-
?
oleic acid + cumene hydroperoxide
-
712359
Avena sativa
cis-9,10-epoxystearic acid + ?
-
-
-
?
thiobenzamide + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
thiobenzamide + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
heme
isoform PXG1 is a hemoprotein
Arabidopsis thaliana
heme
-
Avena sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Arabidopsis thaliana
expressed in Saccharomyces cerevisiae
Avena sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
isoform PXG1 is a hemoprotein
Arabidopsis thaliana
heme
-
Avena sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H138V
the mutant possess only 4% of the heme content wild type isoform PXG1
Arabidopsis thaliana
H70V
the heme content of the mutant is barely measurable (less than 1%) as compared to the wild type isoform PXG1
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
the sulfoxidation of thiobenzamide, catalyzed by isoform PXG1, is completely abolished in the presence of 1 mM 2-mercaptoethanol (competitive inhibitor)
Arabidopsis thaliana
terbufos
a suicide substrate for plant peroxygenases, and at a concentration of 3 mM, it effectively inactivates the activity of isoform PXG1
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Arabidopsis thaliana
16020
-
membrane
integral membrane protein
Avena sativa
16020
-
microsome
-
Arabidopsis thaliana
-
-
microsome
-
Avena sativa
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
the enzyme contains a Ca2+ binding motif, calcium is crucial for its peroxygenase activity
Arabidopsis thaliana
Ca2+
the enzyme contains a Ca2+ binding motif, calcium is crucial for peroxygenase activity
Avena sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
SDS-PAGE
Avena sativa
32000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aniline + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
aniline + cumene hydroperoxide
Avena sativa
-
?
-
-
?
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
linoleic acid + cumene hydroperoxide
Avena sativa
-
?
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
Avena sativa
-
?
-
-
?
oleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
cis-9,10-epoxystearic acid + ?
-
-
?
oleic acid + cumene hydroperoxide
Avena sativa
-
cis-9,10-epoxystearic acid + ?
-
-
?
thiobenzamide + cumene hydroperoxide
Arabidopsis thaliana
-
?
-
-
?
thiobenzamide + cumene hydroperoxide
Avena sativa
-
?
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Arabidopsis thaliana
glycoprotein
-
Avena sativa
sumoylation
isoform PXG1 possesses a high sumoylation potentiality at the position Lys-196
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography
Arabidopsis thaliana
Ni-NTA column chromatography
Avena sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Arabidopsis thaliana
-
seed
-
Avena sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
aniline + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
aniline + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
linoleic acid + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
linoleic acid + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
methyl 4-tolyl sulfide + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
oleic acid + cumene hydroperoxide
-
712359
Arabidopsis thaliana
cis-9,10-epoxystearic acid + ?
-
-
-
?
oleic acid + cumene hydroperoxide
-
712359
Avena sativa
cis-9,10-epoxystearic acid + ?
-
-
-
?
thiobenzamide + cumene hydroperoxide
-
712359
Arabidopsis thaliana
?
-
-
-
?
thiobenzamide + cumene hydroperoxide
-
712359
Avena sativa
?
-
-
-
?
Other publictions for EC 1.11.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742334
Shimada
Leaf oil bodies are subcellul ...
Arabidopsis thaliana
Curr. Opin. Plant Biol.
25
145-150
2015
-
-
-
-
-
-
-
-
1
-
-
3
-
1
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-
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1
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3
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1
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1
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1
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3
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1
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3
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-
-
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1
1
-
-
-
743541
Hanano
Involvement of the caleosin/p ...
Arabidopsis thaliana
Plant Signal. Behav.
10
e991574
2015
-
-
-
-
-
-
-
-
-
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6
-
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1
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1
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1
1
-
-
-
743492
Blee
The reductase activity of the ...
Arabidopsis thaliana
Plant Physiol.
166
109-124
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
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1
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11
1
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1
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1
1
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1
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-
11
1
-
-
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-
-
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-
-
-
1
1
-
-
-
725747
Fuchs
-
Epoxidation, hydroxylation and ...
Solanum lycopersicum
J. Mol. Catal. B
96
52-60
2013
-
-
1
-
-
-
-
3
-
-
2
-
-
1
-
-
-
-
-
-
-
-
9
1
1
-
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-
1
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1
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1
1
-
-
-
-
-
-
3
-
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2
-
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9
1
1
-
-
-
1
-
-
-
-
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-
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-
-
724973
Blee
A non-canonical caleosin from ...
Arabidopsis thaliana
FEBS J.
279
3981-3995
2012
-
-
1
-
-
-
-
-
1
1
1
3
-
4
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
1
1
3
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726143
Kim
A stress-responsive caleosin-l ...
Arabidopsis thaliana
Plant Cell Physiol.
52
874-884
2011
-
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1
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-
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3
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2
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1
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-
2
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
726222
Meesapyodsuk
A peroxygenase pathway involve ...
Avena sativa
Plant Physiol.
157
454-463
2011
-
-
1
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-
-
-
-
2
-
-
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1
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6
-
1
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1
1
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1
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2
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6
-
1
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-
-
1
1
-
-
-
-
-
-
-
-
713295
Partridge
Roles of a membrane-bound cale ...
Arabidopsis thaliana
Plant Physiol. Biochem.
47
796-806
2009
-
-
-
-
-
-
-
-
3
1
2
1
-
4
-
1
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6
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1
1
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3
1
2
1
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1
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6
-
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1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712256
Piazza
One-pot synthesis of fatty aci ...
Avena sativa
J. Am. Oil Chem. Soc.
83
1021-1025
2006
-
-
-
-
-
-
-
-
-
1
-
4
-
1
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1
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4
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1
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1
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4
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1
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4
-
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-
1
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-
-
712359
Hanano
Plant seed peroxygenase is an ...
Arabidopsis thaliana, Avena sativa
J. Biol. Chem.
281
33140-33151
2006
-
-
2
-
2
-
2
-
4
2
2
10
-
7
-
3
2
-
-
3
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
2
-
-
2
-
-
4
2
2
10
-
-
3
2
-
3
-
-
10
-
-
-
-
-
-
-
-
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-
-
671684
Magri
-
Immobilization of soybean seed ...
Glycine max
Biocatal. Biotransform.
23
339-346
2005
-
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1
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1
1
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1
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1
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2
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1
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1
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1
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1
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2
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712357
Kandel
Cloning, functional expression ...
Triticum aestivum
J. Biol. Chem.
280
35881-35889
2005
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
712255
Piazza
Synthesis of a fatty tetrahydr ...
Avena sativa
J. Am. Oil Chem. Soc.
81
933-937
2004
-
-
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-
-
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1
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1
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1
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1
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1
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-
712776
Piazza
-
Epoxidation of fatty acids, fa ...
Avena sativa
J. Mol. Catal. B
21
143-151
2003
-
-
-
-
-
-
-
-
1
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-
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1
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1
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15
-
-
-
-
-
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1
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-
1
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1
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1
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15
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
713275
Lequeu
Formation of plant cuticle: ev ...
Avena sativa, Glycine max, Zea mays
Plant J.
36
155-164
2003
-
-
-
-
-
-
18
-
-
-
-
1
-
3
-
-
-
-
-
3
-
-
6
-
-
-
-
-
-
-
-
-
3
-
15
-
-
-
-
-
-
-
15
18
3
-
-
-
-
1
-
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