KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
indole | partially purified enzyme, using linoleic acid hydroperoxide as cosubstrate, at pH 7.2 and 25°C | Pisum sativum | |
0.08 | - |
indole | partially purified enzyme, using linoleic acid hydroperoxide as cosubstrate, at pH 7.2 and 25°C | Pisum sativum | |
0.26 | - |
linoleic acid hydroperoxide | partially purified enzyme, at pH 7.2 and 25°C | Pisum sativum | |
1.3 | - |
H2O2 | partially purified enzyme, at pH 7.2 and 25°C | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pisum sativum | 16020 | - |
microsome | - |
Pisum sativum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
3 * 62500 + 1 * 41000, SDS-PAGE | Pisum sativum |
62500 | - |
3 * 62500 + 1 * 41000, SDS-PAGE | Pisum sativum |
230000 | - |
gel filtration | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | peroxygenase exists in nature as a complex with a carotenoid-binding macromolecule | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | 20% (v/v) glycerol remarkably stabilizes the enzyme. The enzyme extracted with cholate or deoxycholate is very stable in the presence of glycerol | Pisum sativum |
Triton X-100 | the solubilized peroxygenase is unstable especially in the presence of Triton X-100 | Pisum sativum |
Tween | the solubilized peroxygenase is unstable especially in the presence of Tween 80 | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAF-cellulose column chromatography and agarose gel column chromatography | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
unpurified microsomal enzyme, at pH 7.2 and 25°C | Pisum sativum |
3.4 | - |
partially purified microsomal enzyme, at pH 7.2 and 25°C | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + H2O2 | - |
Pisum sativum | ? | - |
? | |
indole + linoleic acid hydroperoxide | - |
Pisum sativum | ? | - |
? | |
additional information | peroxygenase exists in nature as a complex with a carotenoid-binding macromolecule | Pisum sativum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 3 * 62500 + 1 * 41000, SDS-PAGE | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
hydrogen peroxide-reducible hemoprotein | - |
Pisum sativum |
peroxygenase, hydroperoxide-dependent hydroxylase | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
partially purified enzyme, using 0.2 mM linoleic acid hydroperoxide as cosubstrate | Pisum sativum |
7.2 | - |
membrane-bound enzyme, using 0.2 mM linoleic acid hydroperoxide as cosubstrate | Pisum sativum |
7.2 | - |
partially purified enzyme, using 1.0 mM H2O2 as cosubstrate | Pisum sativum |
8.7 | - |
membrane-bound enzyme, using 1.0 mM H2O2 as cosubstrate | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the purified enzyme preparation contains 3.2 nmol of protoheme/mg of protein | Pisum sativum |