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Literature summary for 1.11.2.2 extracted from
- Myeloperoxidase-catalyzed oxidation of chloroacetonitrile to cyanide (2004), Toxicol. Lett., 146, 249-257.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-penicillamine | addition of D-penicillamine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (17.2% increase at 5 mM) | Homo sapiens |  |
| glutathione | addition of glutathione significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (24% increase at 5 mM) | Homo sapiens | |
| L-cysteine | addition of L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (45.4% increase at 5 mM) | Homo sapiens | |
| N-acetyl-L-cysteine | addition of N-acetyl-L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (16.3% increase at 5 mM) | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobenzoic acid hydrazine | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by 4-amino benzoic acid hydrazine to 52.6% of control value | Homo sapiens | |
| catalase | addition of the antioxidant enzyme catalase to incubation mixtures results in 56.1% decrease in cyanide release by MPO/H2O2/Cl- system | Homo sapiens | |
| indomethacin | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by indomethacin to 88.9% of control value | Homo sapiens | |
| Sodium azide | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by sodium azide to 43.9% of control value | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.089 | - |
Chloroacetonitrile | at pH 5.5, 37°C, 0.1 mM KCl, 0.5 mM H2O2, 0.15 mM chloroacetonitrile, 5 units/ml MPO, and 0.25 mM NaOCl during an incubation period of 60 min | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cl- + H2O2 + chloroacetonitrile | chloride ion (Cl-) is the dominant electron donor, chloroacetonitrile is activated to cyanide by myeloperoxidase/H2O2/Cl- system in vitro | Homo sapiens | HClO + H2O + cyanide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPO | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
optimum temperature for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
optimum pH for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system | Homo sapiens |
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