Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris and Saccharomyces cerevisiae | Cyclocybe aegerita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.05 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.483 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.49 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.85 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
1.53 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
2.5 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
2.8 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
6.2 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
7.9 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cyclocybe aegerita |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Phenyl Sepharose column chromatography, and Q-Source column chromatography | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
5-nitro-1,3-benzodioxole + H2O2 | - |
Cyclocybe aegerita | 4-nitrocatechol + formic acid + H2O | - |
? | |
benzyl alcohol + H2O2 | - |
Cyclocybe aegerita | benzaldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Cyclocybe aegerita |
Synonyms | Comment | Organism |
---|---|---|
aromatic peroxygenase | - |
Cyclocybe aegerita |
UPO | - |
Cyclocybe aegerita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
121 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
203 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
238 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
307 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
338 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
395 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
498 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
524 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
546 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
676 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cyclocybe aegerita |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
veratryl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
32 | - |
veratryl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
124 | - |
benzyl alcohol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
190 | - |
benzyl alcohol | recombinant enzyme from Pichia pastoris, at pH 7.0 temperature not specified in the publication | Cyclocybe aegerita | |
442 | - |
H2O2 | recombinant enzyme from Pichia pastoris, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
500 | - |
H2O2 | recombinant enzyme from Saccharomyces cerevisiae, with benzyl alcohol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
590 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Pichia pastoris, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
700 | - |
5-nitro-1,3-benzodioxole | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
8200 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Saccharomyces cerevisiae, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
11000 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme from Pichia pastoris, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita |