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Literature summary for 1.11.2.1 extracted from

  • Molina-Espeja, P.; Garcia-Ruiz, E.; Gonzalez-Perez, D.; Ullrich, R.; Hofrichter, M.; Alcalde, M.
    Directed evolution of unspecific peroxygenase from Agrocybe aegerita (2014), Appl. Environ. Microbiol., 80, 3496-3507 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Cyclocybe aegerita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita
0.684
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
1.37
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
1.9
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
5.2
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita B9W4V6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-(4-nitrophenoxy)dodecanoic acid + H2O2
-
 Cyclocybe aegerita ?
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Cyclocybe aegerita oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
2,6-dimethoxyphenol + H2O2
-
 Cyclocybe aegerita ?
-
 ?
5-nitro-1,3-benzodioxole + H2O2
-
 Cyclocybe aegerita 4-nitrocatechol + formic acid + H2O
-
 ?
benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
veratryl alcohol + H2O2
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Cyclocybe aegerita
? x * 35942, calculated from amino acid sequence Cyclocybe aegerita

Synonyms

Synonyms Comment Organism
APO
-
 Cyclocybe aegerita
aromatic peroxygenase
-
 Cyclocybe aegerita
UPO1
-
 Cyclocybe aegerita

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 the enzyme shows 50% activity after 1 h at 53°C Cyclocybe aegerita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
88
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
219
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
221
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita
290
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
329
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
 optimum pH for 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Cyclocybe aegerita
6.5
-
 optimum pH for 5-nitro-1,3-benzodioxole Cyclocybe aegerita
7
-
 optimum pH for 2,6-dimethoxyphenol Cyclocybe aegerita

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cyclocybe aegerita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
174
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
211
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
320
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
8800
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita