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Literature summary for 1.11.1.B2 extracted from

  • Bernhardt, P.; Okino, T.; Winter, J.M.; Miyanaga, A.; Moore, B.S.
    A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis (2011), J. Am. Chem. Soc., 133, 4268-4270.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.B2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
H420F inactive Streptomyces sp.
S427H the mutant shows nearly wild type activity at approximately 70% in the monochlorodimedone assay but is unable to catalyze the conversion of 3 into 4 or 5. Instead, the mutant exclusively catalyzes the formation of 3-based bromohydrins Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Vanadium dependent on Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + bromide + H2O2
-
 Streptomyces sp. (3R)-3-bromo-4a-chloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+
-
 ?
3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + chloride + H2O2
-
 Streptomyces sp. (3R)-3,4a-dichloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+
-
 ?
monochlorodimedone + chloride + H2O2
-
 Streptomyces sp. dichlorodimedone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NapH1
-
 Streptomyces sp.
vanadium-dependent chloroperoxidase
-
 Streptomyces sp.
vanadium-dependent haloperoxidase
-
 Streptomyces sp.

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a highly stereoselective chlorination-cyclization reaction in napyradiomycin antibiotic biosynthesis Streptomyces sp.