Literature summary for 1.11.1.9 extracted from

Badr, H.; El-Baz, A.; Mohamed, I.; Shetaia, Y.; El-Sayed, A.S.A.; Sorour, N.
Bioprocess optimization of glutathione production by Saccharomyces boulardii biochemical characterization of glutathione peroxidase (2021), Arch. Microbiol., 203, 6183-6196 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
6-diazo-5-oxo-L-norleucine	20 mM, 60% residual activity	Saccharomyces boulardii (nom. inval.)
AlCl3	10 mM, 67% residual activity	Saccharomyces boulardii (nom. inval.)
BaCl2	10 mM, 52% residual activity	Saccharomyces boulardii (nom. inval.)
CaCl2	10 mM, 81% residual activity	Saccharomyces boulardii (nom. inval.)
DTNB	20 mM, 46% residual activity	Saccharomyces boulardii (nom. inval.)
EDTA	1 mM, about 40% residual activity, activity is completely restored upon addition of Se+ cations, followed by Na+	Saccharomyces boulardii (nom. inval.)
FeCl3	10 mM, 68% residual activity	Saccharomyces boulardii (nom. inval.)
HgCl2	10 mM, 61% residual activity	Saccharomyces boulardii (nom. inval.)
hydroxylamine	20 mM, 25% residual activity	Saccharomyces boulardii (nom. inval.)
iodoacetate	20 mM, 57% residual activity	Saccharomyces boulardii (nom. inval.)
KCl	10 mM, 45% residual activity	Saccharomyces boulardii (nom. inval.)
MBTH	20 mM, 59% residual activity	Saccharomyces boulardii (nom. inval.)
MgSO4	10 mM, 58% residual activity	Saccharomyces boulardii (nom. inval.)
PMSF	20 mM, 59% residual activity	Saccharomyces boulardii (nom. inval.)

Metals/Ions

Metals/Ions	Comment	Organism	Structure
selenium	-	Saccharomyces boulardii (nom. inval.)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	PAGE	Saccharomyces boulardii (nom. inval.)

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces boulardii (nom. inval.)	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by dialysis (20 kDa membrane cutoff), gel-filtration and ion-exchange chromatography	Saccharomyces boulardii (nom. inval.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
445	-	pH 7, 37°C	Saccharomyces boulardii (nom. inval.)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 glutathione + H2O2	-	Saccharomyces boulardii (nom. inval.)	glutathione disulfide + 2 H2O	-	r
glutathione disulfide + 2 H2O	-	Saccharomyces boulardii (nom. inval.)	2 glutathione + H2O2	-	r
L-cysteine + + H2O2	-	Saccharomyces boulardii (nom. inval.)	cystin + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE	Saccharomyces boulardii (nom. inval.)

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Release 2023.1 (January 2023)