Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.9 extracted from

  • Guo, X.; Yu, Y.; Liu, X.; Zhang, Y.; Guan, T.; Xie, G.; Wei, J.
    Heterologous expression and characterization of human cellular glutathione peroxidase mutants (2014), IUBMB Life, 66, 212-219 .
No PubMed abstract available
Search for more literature for 1.11.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C2S/C78S/C115S/C156S/C202S about 10fold increase in activity compared to wild-type. Mutant displays a typical ping-pong mechanism Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens
0.022
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
0.31
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
0.31
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
selenium selenium-dependent enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07203
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.52
-
 wild-type, pH 7.4, 37°C Homo sapiens
5.28
-
 mutant C2S/C78S/C115S/C156S/C202S, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + H2O2
-
 Homo sapiens glutathione disulfide + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
GPX1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 75% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens
4.8
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
683
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
727.8
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 wild-type Homo sapiens
9
-
 mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.4
-
 26% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
216.7
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
216.7
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
231.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens
231.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens