Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Schistosoma mansoni |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M LiSO4,0.2 M sodium acetate, 24% (w/v) PEG 8000, pH 4.5 for mutant enzyme U43C and 0.2 M NaH2PO4, 0.1 M MES, 32% (w/v) PEG-MME 5000, pH 6.0 for mutant enzyme U43S | Schistosoma mansoni |
Protein Variants | Comment | Organism |
---|---|---|
U43C | inactive | Schistosoma mansoni |
U43S | inactive | Schistosoma mansoni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | Gpx is a selenium-containing enzyme | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19400 | - |
1 * 19400, estimated from SDS-PAGE | Schistosoma mansoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + GSH | Schistosoma mansoni | - |
H2O + GSSG | - |
? | |
tert-butyl hydroperoxide + 2 GSH | Schistosoma mansoni | - |
tert-butyl alcohol + GSSG + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | Q00277 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography and HiTrap SP FF column chromatography | Schistosoma mansoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
integument | - |
Schistosoma mansoni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + GSH | - |
Schistosoma mansoni | H2O + GSSG | - |
? | |
tert-butyl hydroperoxide + 2 GSH | - |
Schistosoma mansoni | tert-butyl alcohol + GSSG + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 19400, estimated from SDS-PAGE | Schistosoma mansoni |
Synonyms | Comment | Organism |
---|---|---|
GPx4 | - |
Schistosoma mansoni |
phospholipid glutathione peroxidase | - |
Schistosoma mansoni |
phospholipid hydroperoxide Gpx | - |
Schistosoma mansoni |