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Literature summary for 1.11.1.7 extracted from
- A heme peroxidase of the ascomyceteous lichen Leptogium saturninum oxidizes high-redox potential substrates (2011), Fungal Genet. Biol., 48, 1139-1145.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Leptogium saturninum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 79000 or 2 * 42000, SDS-PAGE | Leptogium saturninum |
| 79000 | - |
1 * 79000 or 2 * 42000, SDS-PAGE | Leptogium saturninum |
| 81000 | - |
gel filtration | Leptogium saturninum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leptogium saturninum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q Sepharose column chromatography and Mono Q column chromatography | Leptogium saturninum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03 | - |
with Azure B as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 0.1 | - |
with veratryl alcohol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 0.4 | - |
with 4-nitrophenol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 0.5 | - |
crude extract, in sodium malonate or citrate buffer (50 mM, pH 4.5), with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) as substrate, temperature not specified in the publication | Leptogium saturninum |
| 0.8 | - |
with 4-hydroxybenzonitrile as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 5.3 | - |
with 4-hydroxybenzoic acid as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 7 | - |
with Reactive Black 5 as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 17 | - |
with NADH as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 20 | - |
with Reactive Blue 5 as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 53 | - |
with 2,6-dimethoxyphenol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication | Leptogium saturninum |
| 243.3 | - |
after 486.6fold purification, in sodium malonate or citrate buffer (50 mM, pH 4.5), with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) as substrate, temperature not specified in the publication | Leptogium saturninum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-dimethoxyphenol + H2O2 | lowest specific activity | Leptogium saturninum | ? + H2O | - |
? |  |
| 4-hydroxybenzoic acid + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| 4-hydroxybenzonitrile + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| 4-nitrophenol + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| adlerol + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| Azure B + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| additional information | no activity with Mn2+ at pH 4.5 and with H2O2 at pH 3.0 | Leptogium saturninum | ? | - |
? | |
| NADH + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| Reactive Black 5 + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
| Reactive Blue 5 + H2O2 | highest specific activity | Leptogium saturninum | ? | - |
? | |
| reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Leptogium saturninum | oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O | - |
? | |
| veratryl alcohol + H2O2 | - |
Leptogium saturninum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer or dimer | 1 * 79000 or 2 * 42000, SDS-PAGE | Leptogium saturninum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POX | - |
Leptogium saturninum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 4 | the activity of the enzyme for ABTS ranges between 100% and 55% at pH 3.0-4.0 and for 2,6-dimethoxyphenol at pH 2.0-4.0 | Leptogium saturninum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Leptogium saturninum | |
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