General Stability | Organism |
---|---|
the optimal buffer for the assay of purified HRP is 50 mM phosphate buffer (pH 7.5) | Armoracia rusticana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrasonication, ammonium sulfate salt precipitation, and phenyl Sepharose column chromatography | Armoracia rusticana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Armoracia rusticana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.65 | - |
crude enzyme, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C | Armoracia rusticana |
772.3 | - |
after 291fold purification, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C | Armoracia rusticana |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer (pH 7.5) containing 20% (v/v) glycerol, at least 6 months, less than 5% loss of activity | Armoracia rusticana |
0-2°C, 50 mM phosphate buffer (pH 7.5), 48-72 h, 40-45% loss of activity | Armoracia rusticana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorophenol + H2O2 | optimal concentrations of 2-chlorophenol and H2O2 are 0.2 mM and 0.3 mM, respectively | Armoracia rusticana | ? | - |
r | |
guaiacol + H2O2 | optimal concentrations of guaiacol and H2O2 are 0.5 mM and 0.3 mM, respectively | Armoracia rusticana | tetraguaiacol + H2O | - |
r | |
phenol + H2O2 | optimal concentrations of phenol and H2O2 are 0.2 mM and 0.3 mM, respectively | Armoracia rusticana | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Armoracia rusticana |
Synonyms | Comment | Organism |
---|---|---|
HRP | - |
Armoracia rusticana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
optimum temperature using phenol or 2-chlorophenol as substrate | Armoracia rusticana |
30 | - |
optimum temperature using guaiacol as substrate | Armoracia rusticana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
thermal denaturation of isolated HRP occurs at temperatures above 42°C, and the enzyme activity decreases rapidly | Armoracia rusticana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Armoracia rusticana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | although peroxidase activity is comparable in the pH range of 6.0-7.5, significant decline in enzyme activity is observed when the pH is increased to above 8.0 | Armoracia rusticana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Armoracia rusticana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Armoracia rusticana | isoelectric focusing | - |
8.8 |