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Literature summary for 1.11.1.7 extracted from

  • Cardinali, A.; Sergio, L.; Di Venere, D.; Linsalata, V.; Fortunato, D.; Conti, A.; Lattanzio, V.
    Purification and characterization of a cationic peroxidase from artichoke leaves (2007), J. Sci. Food Agric., 87, 1417-1423.
    View publication on PubMed
Search for more literature for 1.11.1.7

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 SDS-PAGE Cynara cardunculus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus P84714 Cynara cardunculus scolymus
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Cynara cardunculus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-25 gel filtration, Red Sepharose CL-6B affinity chromatography, HyperDQ high-performance liquid chromatography, and isoelectrofocusing Cynara cardunculus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Cynara cardunculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the crude extract has a specific activity of 10 units/mg, the 43fold purified enzyme has a specific activity of 86.4 units/mg, one unit of peroxidase activity is defined as the absorbance change at 470 nm per minute using guaiacol assay Cynara cardunculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
 Cynara cardunculus tetraguaiacol + H2O
-
 ?
additional information shows no detectable activity with ascorbic acid or veratryl alcohol Cynara cardunculus ?
-
 ?

Synonyms

Synonyms Comment Organism
ALSP
-
 Cynara cardunculus
cationic peroxidase
-
 Cynara cardunculus
hydrogen donor oxidoreductase
-
 Cynara cardunculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 85 the enzyme shows high stability between 5 and 65°C, but is inactivated after drastic heat treatment (10 min at 85°C) Cynara cardunculus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6
-
 Cynara cardunculus

pI Value

Organism Comment pI Value Maximum pI Value
Cynara cardunculus isoelectric focusing
-
 9