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Literature summary for 1.11.1.7 extracted from
- Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius (2008), Chembiochem, 9, 420-425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | Q55080 | DSM 639 | - |
| Sulfolobus acidocaldarius DSM 639 | Q55080 | DSM 639 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and MonoQ column chromatography | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AmplexRed + cumene hydroperoxide | the initial activity of CYP119 in the presence of cumene hydroperoxide is 5fold higher than that observed with H2O2 | Sulfolobus acidocaldarius | ? | - |
? |  |
| AmplexRed + cumene hydroperoxide | the initial activity of CYP119 in the presence of cumene hydroperoxide is 5fold higher than that observed with H2O2 | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
| AmplexRed + H2O2 | - |
Sulfolobus acidocaldarius | ? + H2O | - |
? | |
| AmplexRed + H2O2 | - |
Sulfolobus acidocaldarius DSM 639 | ? + H2O | - |
? | |
| AmplexRed + tert-butyl hydroperoxide | the initial activity of CYP119 in the presence of tert-butyl hydroperoxide is 2fold higher than that observed with H2O2 | Sulfolobus acidocaldarius | ? | - |
? | |
| AmplexRed + tert-butyl hydroperoxide | the initial activity of CYP119 in the presence of tert-butyl hydroperoxide is 2fold higher than that observed with H2O2 | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP119 | - |
Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
high reactivity at 75°C | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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