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Literature summary for 1.11.1.7 extracted from

  • Ozaki, S.I.; Ortiz de Montellano, P.R.
    Molecular engineering of horseradish peroxidase: thioether sulfoxidation and styrene epoxidation by Phe-41 leucine and threonine mutants (1995), J. Am. Chem. Soc., 117, 7056-7064.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
F41L expressed in Trichoplusia ni cells, full activity Armoracia sp.
F41T expressed in Trichoplusia ni cells, full activity Armoracia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
F41L and F41T-mutant, SDS-PAGE Armoracia sp.

Organism

Organism UniProt Comment Textmining
Armoracia sp.
-
peroxidase C
-

Purification (Commentary)

Purification (Comment) Organism
-
Armoracia sp.

Storage Stability

Storage Stability Organism
-80°C, 50 mM sodium phosphate buffer, pH 7, 4 months, fully active Armoracia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
Armoracia sp. ?
-
?
cis-beta-methylstyrene + H2O2 only F41T-mutant Armoracia sp. cis-beta-methylstyrene oxide + trans-beta-methylstyrene oxide + 1-phenyl-2-propanone + benzaldehyde + H2O
-
?
guaiacol + H2O2
-
Armoracia sp. tetraguaiacol + H2O
-
?
I- + H2O2
-
Armoracia sp. ?
-
?
phenyl cyclopropyl thioether + H2O2
-
Armoracia sp. ?
-
?
phenyl ethyl thioether + H2O2
-
Armoracia sp. ?
-
?
phenyl propyl thioether + H2O2
-
Armoracia sp. ?
-
?
styrene + H2O2 only F41L and F41T-mutant Armoracia sp. styrene oxide + benzaldehyde + phenylacetaldehyde + H2O
-
?
thioanisole + H2O2
-
Armoracia sp. ?
-
?
trans-beta-methylstyrene + H2O2
-
Armoracia sp. trans-beta-methylstyrene oxide + benzaldehyde + H2O
-
?