Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method. Crystal structure of the complex of the catalase with the classical catalase inhibitor 3-amino-1,2,4-triazole is determined at 1.95 A resolution | Mycothermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E316F | kcat/Km is 2fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
E316H | kcat/Km is 2.6fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
H246W | kcat/Km is 5.3fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
I313F | slight increase in kcat/Km as compared to kcat/Km of wild-type enzyme | Mycothermus thermophilus |
I314F | kcat/Km is 5.2fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
L321A | kcat/Km is 1.2fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
V536A | kcat/Km is 1.6fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
V536W | kcat/Km is 5.1fold lower than kcat/Km for wild-type enzyme | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I313F | Mycothermus thermophilus | |
10 | - |
H2O2 | pH 7.0, 60°C, wild-type enzyme | Mycothermus thermophilus | |
11 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme L321A | Mycothermus thermophilus | |
20 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316F | Mycothermus thermophilus | |
33 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316H | Mycothermus thermophilus | |
40 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme H246W | Mycothermus thermophilus | |
50 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I314F | Mycothermus thermophilus | |
67 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536A | Mycothermus thermophilus | |
600 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536W | Mycothermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Mycothermus thermophilus | - |
O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | M4GGR7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.033 | - |
pH 7.0, 60°C, mutant enzyme H246W | Mycothermus thermophilus |
0.085 | - |
pH 7.0, 60°C, mutant enzyme V536W | Mycothermus thermophilus |
0.092 | - |
pH 7.0, 60°C, mutant enzyme I314F | Mycothermus thermophilus |
0.107 | - |
pH 7.0, 60°C, mutant enzyme L321A | Mycothermus thermophilus |
0.163 | - |
pH 7.0, 60°C, mutant enzyme E316H | Mycothermus thermophilus |
0.173 | - |
pH 7.0, 60°C, mutant enzyme E316F | Mycothermus thermophilus |
0.213 | - |
pH 7.0, 60°C, wild-type enzyme | Mycothermus thermophilus |
0.232 | - |
pH 7.0, 60°C, mutant enzyme I313F | Mycothermus thermophilus |
0.236 | - |
pH 7.0, 60°C, mutant enzyme V536A | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Mycothermus thermophilus | O2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Mycothermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
152000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme H246W | Mycothermus thermophilus | |
194000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I314F | Mycothermus thermophilus | |
196000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316F | Mycothermus thermophilus | |
203410 | - |
H2O2 | pH 7.0, 60°C, wild-type enzyme | Mycothermus thermophilus | |
205000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I313F | Mycothermus thermophilus | |
261000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316H | Mycothermus thermophilus | |
274000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme L321A | Mycothermus thermophilus | |
860500 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536A | Mycothermus thermophilus | |
2402000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536W | Mycothermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the homotetramer contains a heme d in each active site | Mycothermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3800 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme H246W | Mycothermus thermophilus | |
3900 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I314F | Mycothermus thermophilus | |
4000 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536W | Mycothermus thermophilus | |
7900 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316H | Mycothermus thermophilus | |
9800 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme E316F | Mycothermus thermophilus | |
12900 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme V536A | Mycothermus thermophilus | |
20300 | - |
H2O2 | pH 7.0, 60°C, wild-type enzyme | Mycothermus thermophilus | |
20500 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme I313F | Mycothermus thermophilus | |
24300 | - |
H2O2 | pH 7.0, 60°C, mutant enzyme L321A | Mycothermus thermophilus |