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Literature summary for 1.11.1.6 extracted from

  • Wang, H.; Tokusige, Y.; Shinoyama, H.; Fujii, T.; Urakami, T.
    Purification and characterization of a thermostable catalase from culture broth of Thermoascus aurantiacus (1998), J. Ferment. Bioeng., 85, 169-173 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
H2O2 inhibitory above 60 mM Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
48
-
H2O2 pH 7.0, 30°C Thermoascus aurantiacus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermoascus aurantiacus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron one iron atom per subunit Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000
-
gel filtration Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-
Thermoascus aurantiacus IF0 9862
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 11% carbohydrates Thermoascus aurantiacus

Purification (Commentary)

Purification (Comment) Organism
from culture broth of cells grown on ethanol Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Thermoascus aurantiacus O2 + 2 H2O
-
?
2 H2O2
-
Thermoascus aurantiacus IF0 9862 O2 + 2 H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 75000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable up to Thermoascus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
107000
-
H2O2 pH 7.0, 30°C Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10
-
Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 13
-
Thermoascus aurantiacus

Cofactor

Cofactor Comment Organism Structure
additional information protein displays absorption maxima at 280 nm, 405 nm and 588 nm Thermoascus aurantiacus

pI Value

Organism Comment pI Value Maximum pI Value
Thermoascus aurantiacus isoelectric focusing
-
4.5