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Literature summary for 1.11.1.6 extracted from
- Properties of a novel periplasmic catalase-peroxidase from Escherichia coli O157:H7 (2004), Arch. Biochem. Biophys., 421, 166-174.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene katP, expression of wild-type and mutant enzymes in strain XL-1 Blue and as His-tagged proteins in BL21(DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stopped-flow kinetics, 25°C, pH 7.0 | Escherichia coli | |
| 3 | - |
H2O2 | peroxidase activity, pH 7.0, 25°C | Escherichia coli |  |
| 27 | - |
H2O2 | catalase activity, pH 7.0, 25°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | enzyme contains heme | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene katP | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | enzyme contains an N-terminal signal peptide comprising residues 24-38, clevage site is between residues 23 and 24 | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| catalase-peroxidase | - |
Escherichia coli |
| KatP | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18000 | - |
H2O2 | catalase activity, pH 7.0, 22°C | Escherichia coli | |
| 26000 | - |
H2O2 | peroxidase activity, pH 7.0, 22°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
aasay at, peroxidase activity | Escherichia coli |
| 7 | - |
assay at, catalase activity | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | peroxidase activity | Escherichia coli |
| 6 | 8 | catalase activity | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Escherichia coli | |
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