Crystallization (Comment) | Organism |
---|---|
the enzyme crystallizes in two different forms obtained at pH 4 and pH 5.3, corresponding to form IN, inactive, and OUT, active. In the form OUT, the calcium binding site is fully occupied by Ca2+, coordinated by seven ligands in a distorted pentagonal bipyramidal geometry, and four water molecules | Marinobacter nauticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
four-step purification protocol | Marinobacter nauticus |
Subunits | Comment | Organism |
---|---|---|
dimer | the dimer is stabilized by hydrophobic interactions between both C-terminal coiled coils of the two monomers. There are also hydrophobic interactions among residues 39-66. The presence of Ca2+ triggers conformational changes, which contribute to stronger interactions within the dimer | Marinobacter nauticus |