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Literature summary for 1.11.1.24 extracted from
- Structural and functional characterization of the glutathione peroxidase-like thioredoxin peroxidase from the fungus Trichoderma reesei (2021), Int. J. Biol. Macromol., 167, 93-100 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Trichoderma reesei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 30% (w/v) PEG 400, 200 mM MgCl2, and 100 mM HEPES pH 7.5 | Trichoderma reesei |
| structure in a reduced state and non-catalytic conformation. The overall fold is similar to the other phospholipid-hydroperoxide glutathione peroxidases | Trichoderma reesei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
H2O2 | at pH 7.2 and 30°C | Trichoderma reesei |  |
| 11.7 | - |
H2O2 | pH 8, 25°C | Trichoderma reesei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | G0RHF8 | - |
- |
| Trichoderma reesei QM6a | G0RHF8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose column chromatography, and Superdex 200 gel filtration | Trichoderma reesei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.5 | - |
substrate tert-butyl hydroperoxide, pH 8, 25°C | Trichoderma reesei |
| 28 | - |
substrate H2O2, pH 8, 25°C | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thioredoxin + H2O2 | - |
Trichoderma reesei | thioredoxin disulfide + 2 H2O | - |
? | |
| 2 thioredoxin + H2O2 | - |
Trichoderma reesei QM6a | thioredoxin disulfide + 2 H2O | - |
? | |
| 2 thioredoxin + tert-butyl hydroperoxide | - |
Trichoderma reesei | thioredoxin disulfide + H2O + tert-butyl alcohol | - |
? | |
| 2 thioredoxin + tert-butyl hydroperoxide | - |
Trichoderma reesei QM6a | thioredoxin disulfide + H2O + tert-butyl alcohol | - |
? | |
| H2O2 + pyrogallol | - |
Trichoderma reesei | ? | - |
? | |
| H2O2 + pyrogallol | - |
Trichoderma reesei QM6a | ? | - |
? | |
| H2O2 + reduced thioredoxin | highest activity | Trichoderma reesei | oxidized thioredoxin + H2O | - |
? | |
| additional information | no activity with glutathione | Trichoderma reesei | ? | - |
- |
|
| additional information | no activity with glutathione | Trichoderma reesei QM6a | ? | - |
- |
|
| tert-butyl hydroperoxide + reduced thioredoxin | - |
Trichoderma reesei | tert-butanol + oxidized thioredoxin + H2O | - |
? | |
| tert-butyl hydroperoxide + reduced thioredoxin | - |
Trichoderma reesei QM6a | tert-butanol + oxidized thioredoxin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 19500, calculated from sequence | Trichoderma reesei |
| homodimer | 2 * 19500, calculated from amino acid sequence | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GPX | - |
Trichoderma reesei |
| thioredoxin peroxidase | - |
Trichoderma reesei |
| TRIREDRAFT_47136 | - |
Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life 36 min | Trichoderma reesei |
| 80 | - |
half-life of 36 min at 80°C | Trichoderma reesei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19 | - |
H2O2 | pH 8, 25°C | Trichoderma reesei | |
| 19 | - |
H2O2 | at pH 7.2 and 30°C | Trichoderma reesei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 12 | broad | Trichoderma reesei |
| 10 | - |
- |
Trichoderma reesei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 12 | the enzyme shows best activity between pH 9.0 and 12.0 | Trichoderma reesei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichoderma reesei | calculated from amino acid sequence | - |
5.6 |
| Trichoderma reesei | calculated from sequence | - |
5.6 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
H2O2 | pH 8, 25°C | Trichoderma reesei | |
| 11.7 | - |
H2O2 | at pH 7.2 and 30°C | Trichoderma reesei | |
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