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Literature summary for 1.11.1.24 extracted from
- Distinct characteristics of two 2-Cys peroxiredoxins of Vibrio vulnificus suggesting differential roles in detoxifying oxidative stress (2012), J. Biol. Chem., 287, 42516-42524.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C171S | complete loss of activity | Vibrio vulnificus |
| C50S | complete loss of activity | Vibrio vulnificus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | 1 mM H2O2 overoxidizes isoform Prx2 at the peroxidatic cysteine and results in the loss of the peroxidase activity | Vibrio vulnificus |  |
| additional information | isoform Prx1 is not overoxidized by H2O2 | Vibrio vulnificus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
H2O2 | pH 7.0, 25°C | Vibrio vulnificus | |
| 0.0079 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus | |
| 0.0087 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus | |
| 0.0326 | - |
H2O2 | pH 7.0, 25°C | Vibrio vulnificus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22168 | - |
x * 22168, calculated | Vibrio vulnificus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio vulnificus | A0A087JKY6 | - |
- |
| Vibrio vulnificus | A0A1W6MAW2 | - |
- |
| Vibrio vulnificus MO6-24/O | A0A087JKY6 | - |
- |
| Vibrio vulnificus MO6-24/O | A0A1W6MAW2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isoform Prx1 is occasionally expressed only in cells exposed to high levels of H2O2 | Vibrio vulnificus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 + NADPH + H+ | isoform Prx2 is able to reduce H2O2 in vitro in the presence of thioredoxin A/thioredoxin reductase as electron donors | Vibrio vulnificus | 2 H2O + 2 NADP+ | - |
? | |
| H2O2 + NADPH + H+ | isoform Prx2 is able to reduce H2O2 in vitro in the presence of thioredoxin A/thioredoxin reductase as electron donors | Vibrio vulnificus MO6-24/O | 2 H2O + 2 NADP+ | - |
? | |
| H2O2 + reduced thioredoxin A | - |
Vibrio vulnificus | 2 H2O + oxidized thioredoxin A | - |
? | |
| H2O2 + reduced thioredoxin A | - |
Vibrio vulnificus MO6-24/O | 2 H2O + oxidized thioredoxin A | - |
? | |
| additional information | alkyl hydroperoxidase subunit F is not able to reduce oxidized isoform Prx2 to reactivate its peroxidase activity | Vibrio vulnificus | ? | - |
? | |
| additional information | alkyl hydroperoxidase subunit F is not able to reduce oxidized isoform Prx2 to reactivate its peroxidase activity | Vibrio vulnificus MO6-24/O | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22168, calculated | Vibrio vulnificus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Prx1 | - |
Vibrio vulnificus |
| Prx2 | - |
Vibrio vulnificus |
| TsaA | - |
Vibrio vulnificus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.098 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus | |
| 2.9 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vibrio vulnificus | calculated | - |
4.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Vibrio vulnificus | isoform Prx1 is occasionally expressed only in cells exposed to high levels of H2O2 | additional information |
| Vibrio vulnificus | isoform Prx2 is induced by trace amounts of H2O2 and thereby residential in cells grown aerobically | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform Prx2 effectively scavenges low levels of peroxides because of its high affinity to H2O2, whereas Prx1 quickly degrades higher levels of peroxides because of its high turnover rate and more efficient reactivation | Vibrio vulnificus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus | |
| 88 | - |
H2O2 | pH 7.0, 25°C | Vibrio vulnificus | |
| 330 | - |
reduced thioredoxin A | pH 7.0, 25°C | Vibrio vulnificus |
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