Literature summary for 1.11.1.24 extracted from

DAmbrosio, K.; Limauro, D.; Pedone, E.; Galdi, I.; Pedone, C.; Bartolucci, S.; De Simone, G.
Insights into the catalytic mechanism of the Bcp family: Functional and structural analysis of Bcp1 from Sulfolobus solfataricus (2009), Proteins Struct. Funct. Bioinform., 76, 995-1006.

Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes are overproduced in soluble form as fusion with a C-terminal eight-residue histidine tag in Escherichia coli BL21	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method, crystal structure at 2.15 A, resolution of the C45S/C50S double mutant	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
C45S	mutation in cysteine 45 results in complete loss of DTT-dependent peroxidase activity	Saccharolobus solfataricus
C50S	the C50S mutant enzyme retains almost all the activity of the wild-type enzyme (DTT-dependent peroxidase activity). Unlike the wild-type enzyme, the mutant enzyme is able to form in vitro a homodimer via an intermolecular disulfide bond	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18525	-	x * 18525, calculated from sequence	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97WP9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2O2 + dithiothreitol	-	Saccharolobus solfataricus	H2O + oxidized dithiothreitol	-	?

Subunits

Subunits	Comment	Organism
?	x * 18525, calculated from sequence	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
DTT-dependent peroxidase	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Saccharolobus solfataricus

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Release 2023.1 (January 2023)