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Literature summary for 1.11.1.24 extracted from
- Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum (2007), Plant Physiol. Biochem., 45, 729-739.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | stimulates enzyme expression | Pisum sativum |  |
| Na+ | Na+ at 25 mM stimulates expression of PrxII F | Pisum sativum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Pisum sativum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C48S | 12% activity compared to the wild type enzyme | Pisum sativum |
| C59S | inactive | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0186 | - |
H2O2 | wild type enzyme, in 25 mM Tris-HCl (pH 8) | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Pisum sativum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18570 | - |
estimated from amino acid sequence | Pisum sativum |
| 18750 | - |
mature enzyme, gel filtration | Pisum sativum |
| 20000 | - |
2 * 20000, SDS-PAGE | Pisum sativum |
| 40000 | - |
SDS-PAGE | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | A0ZXY5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
| root | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 + reduced thioredoxin | - |
Pisum sativum | H2O + oxidized thioredoxin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 20000, SDS-PAGE | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PrxII F | - |
Pisum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.6 | - |
H2O2 | wild type enzyme, in 25 mM Tris-HCl (pH 8) | Pisum sativum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Pisum sativum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | estimated from amino acid sequence | - |
5.92 |
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