Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arenicola marina |
Protein Variants | Comment | Organism |
---|---|---|
C183S | strongly reduced activity | Arenicola marina |
C45S | inactive | Arenicola marina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
25000 | - |
1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer | Arenicola marina |
50000 | - |
2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arenicola marina | Q1AN22 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ column chromatography | Arenicola marina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall | - |
Arenicola marina | - |
gill | - |
Arenicola marina | - |
additional information | no activity is detected in muscles, coelomic fluid, and other internal structures | Arenicola marina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cumene hydroperoxide + reduced thioredoxin | - |
Arenicola marina | 2-phenylpropan-2-ol + oxidized thioredoxin + H2O | - |
? | |
tert-butyl hydroperoxide + reduced dithiothreitol | - |
Arenicola marina | tert-butanol + oxidized dithiothreitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
monomer | 1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
monomer | 1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer | Arenicola marina |
Synonyms | Comment | Organism |
---|---|---|
peroxiredoxin 6 | - |
Arenicola marina |
Prdx6 | classified as a 2-Cys PRDX | Arenicola marina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Arenicola marina |