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Literature summary for 1.11.1.24 extracted from

  • Aran, M.; Caporaletti, D.; Senn, A.M.; Tellez de Inon, M.T.; Girotti, M.R.; Llera, A.S.; Wolosiuk, R.A.
    ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin (2008), FEBS J., 275, 1450-1463.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Brassica napus

Protein Variants

Protein Variants Comment Organism
C53S autophosphorylation of C53S 2-Cys Prx does not require successive incubation with dithiothreitol and the hydroperoxide but is extremely sensitive to the addition of dithiothreitol Brassica napus
W179F neither the basal nor the ATP-inhibited peroxidase activities are appreciably different from wild type 2-Cys Prx Brassica napus
Y166F autophosphorylation is similar to wild-type 2-Cys Prx Brassica napus

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibitory at 2 mM Brassica napus
AMP inhibitory at 2 mM Brassica napus
ATP 3 mM ATP in concert with Mg2+, Ca2+, Mn2+, or Zn2+ lowers the peroxidase activity in a dose-dependent manner Brassica napus
Ca2+ 92% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus
Mg2+ in the presence of 2 mM Mg2+, the rate of H2O2 removal is inhibited by 60%, 5% and 5% when it is assayed with 2 mM ADP, AMP or phosphate, respectively, total inactivation of 2-Cys Prx is caused by incubation with 3 mM ATP and 3 mM Mg2+ Brassica napus
Mn2+ 85% 92% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus
phosphate inhibitory at 2 mM Brassica napus
Zn2+ 65% inhibition at 2 mM, in the presence of 3 mM ATP Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
10 * 26000, gel filtration Brassica napus
260000
-
gel filtration Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus Q9FUC5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein ATP facilitates the autophosphorylation of 2-Cys Prx when the protein is successively reduced with thiol-bearing compounds and oxidized with hydroperoxides or quinones Brassica napus

Purification (Commentary)

Purification (Comment) Organism
Ni2+-iminodiacetate-Sepharose column chromatography Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 + reduced thioredoxin
-
Brassica napus H2O + oxidized thioredoxin
-
?

Subunits

Subunits Comment Organism
decamer 10 * 26000, gel filtration Brassica napus

Synonyms

Synonyms Comment Organism
2-Cys peroxiredoxin
-
Brassica napus
2-Cys Prx
-
Brassica napus

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Brassica napus