Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Brassica napus |
Protein Variants | Comment | Organism |
---|---|---|
C53S | autophosphorylation of C53S 2-Cys Prx does not require successive incubation with dithiothreitol and the hydroperoxide but is extremely sensitive to the addition of dithiothreitol | Brassica napus |
W179F | neither the basal nor the ATP-inhibited peroxidase activities are appreciably different from wild type 2-Cys Prx | Brassica napus |
Y166F | autophosphorylation is similar to wild-type 2-Cys Prx | Brassica napus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibitory at 2 mM | Brassica napus | |
AMP | inhibitory at 2 mM | Brassica napus | |
ATP | 3 mM ATP in concert with Mg2+, Ca2+, Mn2+, or Zn2+ lowers the peroxidase activity in a dose-dependent manner | Brassica napus | |
Ca2+ | 92% inhibition at 2 mM, in the presence of 3 mM ATP | Brassica napus | |
Mg2+ | in the presence of 2 mM Mg2+, the rate of H2O2 removal is inhibited by 60%, 5% and 5% when it is assayed with 2 mM ADP, AMP or phosphate, respectively, total inactivation of 2-Cys Prx is caused by incubation with 3 mM ATP and 3 mM Mg2+ | Brassica napus | |
Mn2+ | 85% 92% inhibition at 2 mM, in the presence of 3 mM ATP | Brassica napus | |
phosphate | inhibitory at 2 mM | Brassica napus | |
Zn2+ | 65% inhibition at 2 mM, in the presence of 3 mM ATP | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
10 * 26000, gel filtration | Brassica napus |
260000 | - |
gel filtration | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | Q9FUC5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | ATP facilitates the autophosphorylation of 2-Cys Prx when the protein is successively reduced with thiol-bearing compounds and oxidized with hydroperoxides or quinones | Brassica napus |
Purification (Comment) | Organism |
---|---|
Ni2+-iminodiacetate-Sepharose column chromatography | Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + reduced thioredoxin | - |
Brassica napus | H2O + oxidized thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 26000, gel filtration | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
2-Cys peroxiredoxin | - |
Brassica napus |
2-Cys Prx | - |
Brassica napus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Brassica napus |