Literature summary for 1.11.1.24 extracted from

Dombrecht, B.; Heusdens, C.; Beullens, S.; Verreth, C.; Mulkers, E.; Proost, P.; Vanderleyden, J.; Michiels, J.
Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin (2005), Mol. Microbiol., 55, 1207-1221.

Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rhizobium etli

Protein Variants

Protein Variants	Comment	Organism
C156S	no peroxidase activity	Rhizobium etli
C56S	no peroxidase activity	Rhizobium etli
C81S	although the PrxS C81S mutant protein can be overexpressed and purified under denaturing conditions, it is not possible to obtain any active C81S PrxS. the C81S mutant is prone to inclusion body formation	Rhizobium etli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.062	-	H2O2	-	Rhizobium etli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20080	-	x * 20080, calculated from sequence	Rhizobium etli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rhizobium etli	constitutive expression of prxS confers enhanced survival and growth to Rhizobium etli in presence of H2O2. Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhizobium etli	O69777	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhizobium etli

Source Tissue

Source Tissue	Comment	Organism	Textmining
bacteroid	the enzyme is strongly expressed under microaerobic conditions and during the sambiotic interaction with Phaseolus vulgaris	Rhizobium etli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2O2 + dithiothreitol	-	Rhizobium etli	H2O + oxidized dithiothreitol	-	?
H2O2 + reduced thioredoxin	-	Rhizobium etli	H2O + oxidized thioredoxin	-	?
additional information	constitutive expression of prxS confers enhanced survival and growth to Rhizobium etli in presence of H2O2. Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin	Rhizobium etli	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 20080, calculated from sequence	Rhizobium etli

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rhizobium etli	calculated from sequence	-	5.3

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Release 2023.1 (January 2023)