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Literature summary for 1.11.1.24 extracted from

  • Sekiya, M.; Mulcahy, G.; Irwin, J.A.; Stack, C.M.; Donnelly, S.M.; Xu, W.; Collins, P.; Dalton, J.P.
    Biochemical characterisation of the recombinant peroxiredoxin (FhePrx) of the liver fluke, Fasciola hepatica (2006), FEBS Lett., 580, 5016-5022.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0127
-
cumene hydroperoxide 22°C, pH 7.0 Fasciola hepatica
0.0186
-
tert-butyl hydroperoxide 22°C, pH 7.0 Fasciola hepatica
0.03
-
H2O2 22°C, pH 7.0 Fasciola hepatica

Organism

Organism UniProt Comment Textmining
Fasciola hepatica P91883
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Fasciola hepatica

Reaction

Reaction Comment Organism Reaction ID
2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH the enzyme exhibits a saturable, single-displacement-like reaction mechanism rather than non-saturable double displacement (ping-pong) enzyme substitution mechanism Fasciola hepatica

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cumene hydroperoxide + reduced thioredoxin
-
Fasciola hepatica ?
-
?
H2O2 + reduced thioredoxin
-
Fasciola hepatica H2O + oxidized thioredoxin
-
?
tert-butyl hydroperoxide + reduced thioredoxin
-
Fasciola hepatica ?
-
?

Synonyms

Synonyms Comment Organism
2-Cys peroxiredoxin
-
Fasciola hepatica
FhePrx
-
Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3
-
cumene hydroperoxide 22°C, pH 7.0 Fasciola hepatica
7.6
-
tert-butyl hydroperoxide 22°C, pH 7.0 Fasciola hepatica
15.8
-
H2O2 22°C, pH 7.0 Fasciola hepatica