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Literature summary for 1.11.1.24 extracted from

  • Huang, J.K.; Ken, C.F.; Huang, H.M.; Lin, C.T.
    Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata (2007), Appl. Microbiol. Biotechnol., 74, 84-92.
    View publication on PubMed
Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment) Organism
coding region is subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein Taiwanofungus camphoratus

General Stability

General Stability Organism
susceptible to chymotrypsin treatment but resistant to digestion by trypsin. The enzyme retains approximately 65%, 50%, and 40% activity after treating with chymotrypsin for 1, 2, and 3 h, respectively Taiwanofungus camphoratus

Inhibitors

Inhibitors Comment Organism Structure
imidazole 0.1 M, 50% inhibition Taiwanofungus camphoratus
SDS 1%, slight decrease in activity Taiwanofungus camphoratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20965
-
 2 * 20965, calculated from sequence Taiwanofungus camphoratus
21000
-
 2 * 21000, SDS-PAGE Taiwanofungus camphoratus

Organism

Organism UniProt Comment Textmining
Taiwanofungus camphoratus A1KXU3 i.e. Taiwanofungus camphoratus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Taiwanofungus camphoratus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Taiwanofungus camphoratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 + dithiothreitol
-
 Taiwanofungus camphoratus H2O + oxidized dithiothreitol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 21000, SDS-PAGE Taiwanofungus camphoratus
dimer 2 * 20965, calculated from sequence Taiwanofungus camphoratus

Synonyms

Synonyms Comment Organism
2-Cys Prx
-
 Taiwanofungus camphoratus
thioredoxin peroxidase
-
 Taiwanofungus camphoratus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 2 min, Prx retains 68% activity Taiwanofungus camphoratus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 37°C, 30 min, stable Taiwanofungus camphoratus

pI Value

Organism Comment pI Value Maximum pI Value
Taiwanofungus camphoratus calculated from sequence
-
 5.9