Any feedback?
Literature summary for 1.11.1.24 extracted from
- Peroxiredoxin-linked detoxification of hydroperoxides in Toxoplasma gondii (2005), J. Biol. Chem., 280, 564-570.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| TgTrx-Px1 and TgTrx-Px2, expression in Escherichia coli | Toxoplasma gondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tert-butyl hydroperoxide | substrate inhibition | Toxoplasma gondii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
tert-butyl hydroperoxide | - |
Toxoplasma gondii | |
| 0.00038 | - |
H2O2 | - |
Toxoplasma gondii | |
| 0.0042 | - |
thioredoxin | - |
Toxoplasma gondii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Toxoplasma gondii | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24600 | - |
10 * 24600, TgTrx-Px1, SDS-PAGE | Toxoplasma gondii |
| 25000 | - |
TgTrx-Px2 monomer, gel filtration | Toxoplasma gondii |
| 26500 | - |
1 * 26500, TgTrx-Px2, SDS-PAGE | Toxoplasma gondii |
| 52000 | - |
TgTrx-Px2 dimer, gel filtration | Toxoplasma gondii |
| 150000 | - |
TgTrx-Px2 hexamer, gel filtration | Toxoplasma gondii |
| 250000 | - |
TgTrx-Px1 decamer, gel filtration | Toxoplasma gondii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Toxoplasma gondii | peroxidases belonging to the class of 1-Cys and 2-Cys peroxiredoxins play crucial roles in maintaining redox balance. TgTrx-Px1 is an extremely potent antioxidant | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Toxoplasma gondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tachyzoite | - |
Toxoplasma gondii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 + reduced thioredoxin | ping-pong mechanism | Toxoplasma gondii | H2O + oxidized thioredoxin | - |
? | |
| additional information | peroxidases belonging to the class of 1-Cys and 2-Cys peroxiredoxins play crucial roles in maintaining redox balance. TgTrx-Px1 is an extremely potent antioxidant | Toxoplasma gondii | ? | - |
? | |
| tert-butyl hydroperoxide + reduced thioredoxin | - |
Toxoplasma gondii | tert-butanol + oxidized thioredoxin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | 10 * 24600, TgTrx-Px1, SDS-PAGE | Toxoplasma gondii |
| dimer | 1 * 26500, TgTrx-Px2, SDS-PAGE | Toxoplasma gondii |
| hexamer | 1 * 26500, TgTrx-Px2, SDS-PAGE | Toxoplasma gondii |
| monomer | 1 * 26500, TgTrx-Px2, SDS-PAGE | Toxoplasma gondii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-Cys peroxiredoxin | - |
Toxoplasma gondii |
| 2-Cys peroxiredoxin | - |
Toxoplasma gondii |
| TgTrx-Px1 | - |
Toxoplasma gondii |
| TgTrx-Px2 | - |
Toxoplasma gondii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
H2O2 | - |
Toxoplasma gondii | |
| 2.79 | - |
tert-butyl hydroperoxide | - |
Toxoplasma gondii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
