BRENDA - Enzyme Database show
show all sequences of 1.11.1.23

Protein purification and function assignment of the epoxidase catalyzing the formation of fosfomycin

Liu, P.; Murakami, K.; Seki, T.; He, X.; Yeung, S.M.; Kuzuyama, T.; Seto, H.; Liu, H.; J. Am. Chem. Soc. 123, 4619-4620 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Streptomyces wedmorensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
iron-dependent enzyme, one iron per monomer
Streptomyces wedmorensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21210
-
4 * 21210, calculated from sequence
Streptomyces wedmorensis
89000
-
gel filtration
Streptomyces wedmorensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces wedmorensis
Q56185
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces wedmorensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-hydroxypropylphosphonic acid + 2 NADH + O2
-
704155
Streptomyces wedmorensis
cis-(1R,2S)-epoxypropylphosphonic acid + 2 H2O + 2 NAD+
i.e. fosfomycin
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 21210, calculated from sequence
Streptomyces wedmorensis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
FMN and FAD greatly enhance production of fosfomycin. The flavin coenzyme is unlikely an integral part of the epoxidase
Streptomyces wedmorensis
FMN
FMN and FAD greatly enhance production of fosfomycin. The flavin coenzyme is unlikely an integral part of the epoxidase
Streptomyces wedmorensis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Streptomyces wedmorensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
FMN and FAD greatly enhance production of fosfomycin. The flavin coenzyme is unlikely an integral part of the epoxidase
Streptomyces wedmorensis
FMN
FMN and FAD greatly enhance production of fosfomycin. The flavin coenzyme is unlikely an integral part of the epoxidase
Streptomyces wedmorensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
iron-dependent enzyme, one iron per monomer
Streptomyces wedmorensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21210
-
4 * 21210, calculated from sequence
Streptomyces wedmorensis
89000
-
gel filtration
Streptomyces wedmorensis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces wedmorensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-hydroxypropylphosphonic acid + 2 NADH + O2
-
704155
Streptomyces wedmorensis
cis-(1R,2S)-epoxypropylphosphonic acid + 2 H2O + 2 NAD+
i.e. fosfomycin
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 21210, calculated from sequence
Streptomyces wedmorensis
Other publictions for EC 1.11.1.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729898
Huang
Mechanistic consequences of ch ...
Streptomyces wedmorensis
J. Am. Chem. Soc.
136
2944-2947
2014
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727328
Milaczewska
On the catalytic mechanism of ...
Streptomyces wedmorensis
Chemistry
19
771-781
2013
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727696
Chang
Reaction of HppE with substrat ...
Streptomyces wedmorensis
J. Am. Chem. Soc.
135
8153-8156
2013
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17
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730966
Wang
Evidence that the fosfomycin-p ...
Yersinia pseudotuberculosis
Science
342
991-995
2013
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727675
Huang
Evidence for radical-mediated ...
Streptomyces wedmorensis
J. Am. Chem. Soc.
134
16171-16174
2012
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1
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8
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702244
Munos
Purification and characterizat ...
Pseudomonas syringae
Biochemistry
47
8726-8735
2008
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1
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702547
Schweifer
On the conversion of structura ...
Streptomyces fradiae
Bioorg. Med. Chem. Lett.
18
3056-3059
2008
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1
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702211
Yan
Determination of the substrate ...
Streptomyces wedmorensis
Biochemistry
46
12628-12638
2007
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702208
Yan
Biosynthesis of fosfomycin, re ...
Streptomyces wedmorensis
Biochemistry
45
11473-11481
2006
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701481
Cameron
Initiating a crystallographic ...
Streptomyces wedmorensis
Acta Crystallogr. Sect. F
61
534-536
2005
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701900
Yan
Site-directed mutagenesis and ...
Streptomyces wedmorensis
Arch. Biochem. Biophys.
442
82-91
2005
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1
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3
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705876
Higgins
Structural insight into antibi ...
Streptomyces wedmorensis
Nature
437
838-844
2005
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706480
McLuskey
Structure and reactivity of hy ...
Streptomyces wedmorensis
Proc. Natl. Acad. Sci. USA
102
14221-14226
2005
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704157
Liu
Oxygenase activity in the self ...
Streptomyces wedmorensis
J. Am. Chem. Soc.
126
10306-10312
2004
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3
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702205
Liu
Biochemical and spectroscopic ...
Streptomyces wedmorensis
Biochemistry
42
11577-11586
2003
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701694
Zhao
Mechanistic studies of HPP epo ...
Streptomyces wedmorensis
Angew. Chem. Int. Ed. Engl.
41
4529-4532
2002
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704155
Liu
Protein purification and funct ...
Streptomyces wedmorensis
J. Am. Chem. Soc.
123
4619-4620
2001
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701777
Watanabe
A phosphonate-induced gene whi ...
Penicillium decumbens
Appl. Environ. Microbiol.
65
1036-1044
1999
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702728
Kuzuyama
Cloning and expression in Esch ...
Pseudomonas syringae, Pseudomonas syringae PB-5123
Biosci. Biotechnol. Biochem.
63
2222-2224
1999
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705742
Hidaka
Cloning and nucleotide sequenc ...
Streptomyces wedmorensis 144-91, Streptomyces wedmorensis
Mol. Gen. Genet.
249
274-280
1995
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