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Literature summary for 1.11.1.21 extracted from

  • Vidossich, P.; Loewen, P.C.; Carpena, X.; Fiorin, G.; Fita, I.; Rovira, C.
    Binding of the antitubercular pro-drug isoniazid in the heme access channel of catalase-peroxidase (KatG). A combined structural and metadynamics investigation (2014), J. Phys. Chem. B, 118, 2924-2931 .
    View publication on PubMed
Search for more literature for 1.11.1.21

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme D141A in complex with isoniazid, hanging drop vapor diffusion method, using 16-20% (w/v) PEG 4000, 20% (w/v) 2-methyl-2,4-pentanediol, and 0.1 M sodium citrate pH 5.6 Burkholderia pseudomallei

Protein Variants

Protein Variants Comment Organism
A143Q the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
A143V the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
A290Q the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
A290Y the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
D141A the mutant shows reduced catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
E242Q the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
L209D the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
L236D the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
Q233E the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei
W309F the mutant shows unchanged catalase and peroxidase activities compared to the wild type enzyme Burkholderia pseudomallei

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei Q3JNW6
-
-
Burkholderia pseudomallei 1710b Q3JNW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isoniazid + H2O2
-
 Burkholderia pseudomallei ?
-
 ?
isoniazid + H2O2
-
 Burkholderia pseudomallei 1710b ?
-
 ?

Synonyms

Synonyms Comment Organism
KatG
-
 Burkholderia pseudomallei

Cofactor

Cofactor Comment Organism Structure
heme
-
 Burkholderia pseudomallei